Wenyang Huoxue Lishui Fang To Arab League Mildew Element Nephrosis Big Mouse Renal Glomerulus Supporting Cell Podocin Expression Influence

Objective:Observation Wenyang Huoxue Lishui Fang on adriamycin-induced nephropathy rat glomerular podocyte specific podocin protein expression.Discusses the Wenyang Huoxue Lishui Fang initially to the nephrosis syndrome therapeutic action.Methods:SPF level 50 male Wistar rats,weight 250±20 grams, were randomly divided into 4 groups.5 normal group,model group 15,15 prednisone group,the Chinese Medicines Board 15.Except the normal group,other groups are adriamycin(ADR) 5.5mg/kg disposable tail vein injection,induced a similar human minimal change nephropathy in rat kidney model,the normal group disposable tail vein injection 1 ml normal saline.Beginning one week after injection of ADR treatment,the Chinese Medicines Board once a day by gastric garage Wenyang Huoxue Lishui Fang 3ml,prednisone group by 12mg/kg·d,by gastric gavage three milliliters suspension prednisone(prednisone dose with the former),the normal group and model group daily gavage three milliliters of normal saline,were sustained for 4 weeks.Experimental observation of rat faeces,urine,body weight,edema,tongue,such as the general situation;model before the model No.1 weekend,3 weekends and 5 were measured in rats over the weekend 24-hour urinary protein;experimental section 5 over the weekend derived rats were killed.(1) After the anaesthesia,draws blood 5 ml in the heart,the separation of serum,detection of total protein(TP),albumin(ALB),total cholesterol(CHOL),triglyceride(TG),low density lipoprotein (LDC-C) level.(2) Using immunofluorescent staining and RT-PCR semi-quantitative determination of each group in the rat glomerular podocyte expression level of podocin.(3) Access to renal tissue by light microscopy Histomorphology Detect Change; electron microscope detection of ultrastructural changes.Results:(1) Ordinary circumstances:The normal group big mouse hair has the gloss,moves many,the state of mind is good,the appetite is good.Other each group of big mice inject the Arab League mildew element 4～5 days later the big mouse starts to present the body weight to drop,the slow reaction,the activity reduces,the back rolls up,gathers piles,the pili is sparse, even the laminated shape falls off,feed and potable water obvious reduction,then pond.Injects the Arab League mildew element 6～7 days later the big mouse starts to present four podoedemas,the foot,the tail reddish purple,the tongue substance purple dark.Makes mold period because has 2 big mice the serious dropsy death.And model group and prednisone group each.Each treatment group uses the traditional Chinese medicine,the prednisone e treat 1 week later the big mouse to respond separately changes for the better gradually,before the feed and the potable water quantity increase,the activity increases;And the traditional Chinese medicine group amount of urine increases obviously,the body weight rises again slowly.The prednisone group big mouse performance for exceptionally moves restlessly,aggressive.(2) In urine and blood test results:Makes the mold latter 1 week,makes the mold train big mouse 24 hour urine protein quota and the injection,before respectively is quite even obviously elevates.Wenyang Huoxue Lishui Fang and prednisone treats 4 weeks later can obviously lower the Arab League mildew element nephrosis big mouse's urine protein level.But between traditional Chinese medicine group and prednisone group difference non-statistics significance.Arab League mildew element nephrosis big mouse existence obvious low proteinemia and the hyperlipemia,Wenyang Huoxue Lishui Fang and prednisone can treats 4 weeks later be able to elevate the Arab League mildew element nephrosis big mouse's serum albumin level.But only Wenyang Huoxue Lishui Fang can lower its the blood serum lipin level.(3) Immunofluorescence staining and RT-PCR Results:Arab League mildew element nephrosis big mouse renal glomerulus supporting cell podocin mRNA and the protein expression reduces, but Wenyang Huoxue Lishui Fang and the prednisone can reduce podocin mRNA and the protein expression drop to a certain extent.(4) Pathology:Wenyang Huoxue Lishui Fang can improve the Arab League mildew element nephrosis big mouse kidney organization's pathology damage to a certain extent.Conclusion:Discovered in the experimental study that the Wenyang Huoxue Lishui Fang can reduce the Arab League mildew element nephrosis big mouse's albuminuria;Control cholesterol, blood fats;Increases the serum albumin;Reduces the Arab League mildew element nephrosis big mouse kidney to organize the podocin expression the drop;Reduces it's the kidney organization's pathology damage...