Expression Of HBsAg In Human Oocytes And Early Embryos

Background:Hepatitis B virus infection is a global health problems,the main epidemic areas were in Asia,Africa,southern Europe and Latin America.In the world,there are 200 millions people in the past or now have the serology evidence who has a persistent infection of hepatitis B virus.In the world,there have 35 millions chronic HBV infection,acute and chronic hepatitis,cirrhosis,liver cancer are closely related to HBV. About 15～25%of them have died of HBV-related end-stage liver cirrhosis and liver cancer.Every year＞1 millions died of acute and chronic HBV infection,China has 17 millions of chronic HBV-infected persons.HBV transmited mainly through blood, mother to child transmission,sexual contact and close contact.Domestic chronic HBsAg carriers,about 40%through mother to child transmission,some baby had infected by HBV in the uterus,intrauterine infection rate of 5～10%.Vertical transmission of HBV infection in China is an important form.The broad vertical transmission was mean transmission between parent and offspring.But the true vertical transmission,meaning that HBV vertically transmits from parent to offspring via affected germ cell through fertilization.According to sex,including mother to offspring and father to offspring vertically transmission.There are more study about human sperm, but vertically transmission from human oocyte was difficult to carry out,mainly due to the oocyte was difficult to get,reseach about human preimplantation embryos wasn't reported.But there are more animal experiments had be done that proved HBV transmits to offspring via oocyte.Objective:Previous experiments has confirmed that HBVDNA existed in the oocytes and early embryos.Next we used immunofluorescence detect expression of HBsAg in oocytes and early embryos,to confirm HBV infect oocytes and its transcriptional activity.,and provide some direct evidence about HBV can transfer to offspring through the oocyte and sperm.Materials and Methods:study groups:Oocytes:IVF patients,woman were HBV carriers,unfertilized oocytes after the operation.Early embryos:at the same time,either husband and wife is HBV carriers,after the operation those early embryos were abandoned.Negative control:at the same time,both husband and wife are not HBV carriers,after the operation,the unfertilized oocytes and early embryos were abandoned.Positive control: Hepatocellular carcinomal cell line.Using Immunofluorescence detect expression of HBsAg in oocytes and early embryos.Results:In the study group we can detected the FITC expression in oocytes and early embryos,and in the negative control group were not detected.The expression of FITC fluorescence in the oocytes are not same;in embryos,there is a single signal in a single cell,there are more signals in a single cell,there are more signals in a number of embryonic cells.Conclusion:HBsAg can expression in human oocytes and early embryos,HBsAg of expression is not uniform.Human oocyte or sperm with HBV-DNA is possibility as a vector to deliver HBV-DNA into early embryo via fertilization.