| [OBJECIIVE]:To investigate Endostatin(Endostatin) mRNA and vascular endothelial growth factor(VEGF) mRNA impact by contrasting endostatin gene recombinant adenovirus vector with mifepristone on rat endometriosis lesions;.[Methods]:Select 40 SD rats with ripe sexual cycle,gain endometrial stripping, suture the stripping on both sides of the peritoneal surface respectively.After 4 weeks,confirm whether or not set up a successful model.Will be successfully established rat model of endometriosis of the SD rats were randomly divided into three groups,each group for endostatin 13,9 mifepristone group and control group 10.Group endostatin endostatin gene recombinant adenovirus vector injection one week,mifepristone mifepristone group were given orally three weeks,the corresponding control group given a placebo,were completed in the fourth week after administration of myocardial blood executed. Specimens stored in -70℃refrigerator,extracting the mRNA samples, the use of Real-time PCR method of detection of both localized lesions Endostatin(Endostatin) and vascular endothelial growth factor(VEGF) mRNA levels,and immunohistochemistry Detect Ways specimens microvessel density(MVD).All data used SAS8.1 statistical software to P＜0.05 as a test,multiple comparisons between groups using Kruskal-Wallis rank sum test and t test,MVD values using analysis of variance(SNK) analysis.[RESULTS]:1,Endostatin-mRNA value in Endostatin group(-△CT) a median of -0.100,-2.695 than mifepristone group and control group of-3.890, compared three groups H value 16.6253,P value was 0.0002,P＜0.01, there is statistical significance;22 comparison,endostatin group compared with the mifepristone group t value of 5.8505,P value was 0.0000,compared with the control group,t value of 5.4303,P value was 0.0001,both P＜0.01 have statistical significance.2,VEGF-mRNA mifepristone group median value of -4.945, significantly less than endostatin group -2.755 and -2.090 in control group among the three groups was compared H value 12.5289,P value of 0.019,P＜0.05,have statistical significance;22 after comparing mifepristone group and endostatin group t value of 4.0920,P value was 0.0007,compared with the control group,t value of-4.1456,P value of 0.0012,both P＜0.01 have statistical significance;3,Three groups Endostatin/VEGF ratio compared endostatin group median value of 2.745,2.010 ratio of mifepristone group,the control group was -1.205,compared among the three groups,H a value of 11.2008,P value was 0.0019,P＜0.01,there is statistical significance. After comparing 22,endostatin group and mifepristone group,tvalue of 2.0351,P value was 0.0568,P＞0.05,no statistical difference. Endostatin group and the control group compared to,tvalue of 4.3060, P value was 0.0006,P＜0.01,there is statistical difference.Mifepristone group and control group,t value of 2.4109,P value was 0.0314,P＜0.05,there is statistical difference.4,MVD values and found that endostatin group and the mifepristone group MVD value than those of the control group there is significant difference between the two groups no significant difference.[CLUSIONS]:1,Endostatin gene recombinant adenovirus vector after intravenous administration of three weeks,rats with lesions of endometriosis localized secretion of endostatin mRNA increased significantly, higher than the mifepristone group and control group.Prompt intravenous administration are endostatin gene recombinant adenovirus vector treatment of endometriosis of the new method.2,Oral mifepristone be able to reduce the rat endometriosis lesions localized secretion of VEGF-mRNA was significantly lower than that of endostatin and control groups.Prompt effects of mifepristone on endometriosis lesions of the anti-angiogenic effect and a lowering of the local secretion of VEGF.3,By the MVD comparation,we found that both mifepristone and endostatin can inhibit endometriosis lesions angiogenesis,which leads to the point—further study of their joint effects should be proposed. |
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