Association Study Between NPPB Gene Polymorphisms And Essential Hypertension In Han Population

Objective: The aim was to investigate the association between NPPB gene and essential hypertension in Han population.Methods: In 120 essential hypertensive and 120 normotensive subjects, -1191G/T polymorphisms of NPPB gene was detected by polymerase chain reaction(PCR) and direct nucleotide sequencing method. Chi-square test was used to analyze qualitative data, t-test or analysis of variance was used to anlyze quantitative data. In essential hypertensive and normotensive subjects,clinical data including age, sex, body mass index(BMI),triglyceride(TG),total cholesterol(TC), creatinine(Cr),uric acid(URIC), heart rate, mean arterial pressure(MAP),pulse pressure(PP) were recorded. The primary hypertensive subjects and normotensive subjects were divided respectively into three groups according to genotype. We observed the distribution of polymorphisms of NPPB gene in Han population.Results: 1.Blood pressure and Cr were significantly different between primary hypertensive and normotensive subjects(P<0.05). 2.The frequencies of different genotypes of NPPB were in agreement with Hardy-Weinberg equilibrium in our study(P>0.05).3.No significant difference was detected in frequencies of genotypes of -1191G/T polymorphisms in NPPB gene in primary hypertensive and normotensive subjects groups. 4. In normotensive subjects, the variant showed a significant on the PP levels by evidenced by the different PP levels between the with different genotypes(P<0.05). 5. In a pregnancy-induced hypertension subject, we found a insert mutation in 5'flanking region of NPPB gene.Conclusion:1. No significant difference was detected in frequencies of genotypes of -1191G/T polymorphisms in NPPB gene in primary hypertensive and normotensive subjects groups.2. In a pregnancy-induced hypertension subject, we found a insert mutation in 5' flanking region of NPPB gene.