Biological Characteristics Of Mesenchymal Stem Cellsderived From Bone Marrow Of Patients With Myelodysplastic Syndrome And With Acute Myeloid Leukemia

Objective To study the difference of biological characteristics between bone marrow(BM)-derived mesenchymal stem cells(MSCs) from patients with myelodysplastic syndrome(MDS) and those with acute myeloid leukemia(AML).Methods 1.MSCs were isolated from BM of different patients(AML with maturation,M2,n=9;acute monocytic leukemia,M5,n=9;myelodysplastic syndrome,MDS,n=7) and cultured in vitro and compared them with MSCs derived from BM of normal adults(n=6).MSCs derived from BM of normal adults were used as the control group.2.The morphology and proliferative property of MSCs from these four sources were observed;3.The immunophenotype of MSCs were detected by fluorescence-activated cell sorter(FACS) and immunocytochemistry.4.BM-MSCs were cultured in soft agar for 2 weeks to observe the clone growth;5.The rate of adhesion of BM-MSCs was detected; 6.Semi-quantitative RT-PCR was used to detect the mRNA expressions of IL-6,SDF-1,SCF and BCL-2 in different BM-MSCs derived from M2,M5 and MDS patients;7.proliferation curves were drawn of K562 cells which were cultivated in suspension and adhesively cultivated respectively with BM-MSCs derived from normal adults,M2,M5 and MDS patients.Results 1.BM-MSCs derived from MDS,M2,M5 patients and normal adults presented a fibroblast-like morphology.2.Doubling of BM-MSCs derived from M2 and M5 patients obviously took more time,compared with BM-MSCs derived from MDS patients and the control group.3.BM-MSCs derived from MDS and AML patients were positive for CD29 and CD44,but negative for CD14 and CD34.4.After two weeks' culture,no clone was formed from BM-MSCs derived from MDS,M2 and M5 patients.5.The rate of adhesion of four kinds of BM-MSCs had no significant differences(P＞0.05).6.MDS,M2 and M5 patients had higher mRNA expression levels of IL-6 and SDF-1 than of the control group(P＜0.01),but in the expression of SCF and BCL-2,there were no significant differences among the control group,MDS,M2 and M5 patients(P＞0.05). Among M2,M5 and MDS patients,the expression of IL-6,SDF-1,SCF and BCL-2 also had no significant differences(P＞0.05).7.K562 cells cultivated with BM-MSCs derived from the control group were inhibited.But the proliferation of K562 cells cultivated with derived from MDS,M2 and M5 patients was accelerated,yet showing no obvious differences in the effect of BM-MSCs from different sources(P＞0.05).Conclusion 1.BM-MSCs derived from M2,M5,MDS patients and normal adults are similar in morphology,immunophenotype and adhesiveness,and they have no oncogenicity.2.MDS,M2 and M5 patients have higher mRNA expression levels of IL-6 and SDF-1 than of the control group,and the proliferation of K562 cells cultivated with BM-MSCs derived from MDS,M2 and M5 patients are accelerated.3.The proliferation capacity of BM-MSCs derived from M2 and M5 patients is lower in comparison with the control group.BM-MSCs derived from MDS patients and the control group are similar in proliferation capacity.