Detection Of K-ras, C-myc And CyclinD1 In Colorectal Adenoma And Colorectal Adenocarcinoma

Objective : To investigate the expression of k-ras,c-myc and cyclinD1 gene in Colorectal adenoma,Colorectal adenocarcinoma and normal Colorectal tissue,explore the effect of k-ras,c-myc and cyclinD1 gene expression on the development and progression in Colorectal adenocarcinoma and .Colorectal adenoma.Method: All cases are from the patients who have received surgery in our hospital.The samples from biopsy with endoscopy or surgery resection are confirmed colorectal adenocarcinoma or adenoma, the colorectal cancer is sporadic excluding Hereditary Non-Polyposis Colorectal Cancer(HNPCC) and Familial Adenomatous Polyposis(FAP).Recording patient's age, sex, susceptive factors to colorectal cancer in details.Expression of k-ras,c-myc and cyclinD1 were assayed by semiquantitive reverse transcriptase polymerase chain reaction(RT-PCR)in 70 cases of Colorectal adenocarcinoma ,36 cases of Colorectal adenoma,10 cases of Malignant transformation of colorectal adenoma organizations and 10 cases of normal Colorectal tissue.Result: The positive rate and expression quantity of k-ras,c-myc and cyclinD1 in colorectal carcinoma were higher than those in Colorectal adenoma (P<0.05) .The positive rate and expression quantity of k-ras,c-myc and cyclinD1 in Poorly differentiated colorectal adenocarcinoma were higher than those in Well-differentiated colorectal adenocarcinoma (P<0.05) .The positive rate and expression quantity of k-ras,c-myc and cyclinD1 in Colorectal adenomas with severe dysplasia were higher than those in Colorectal adenoma with mild atypical hyperplasia (P<0.05) . The expression quantity of k-ras,c-myc and cyclinD1 was correlated with histological grade of colorectal carcinoma,the expression of k-ras,c-myc and cyclinD1 was higher in Poorly differentiated colorectal carcinoma;The expression quantity of k-ras,c-myc and cyclinD1 was correlated with The degree of hyperplasia of Colorectal adenoma,the expression of k-ras,c-myc and cyclinD1 was higher in Colorectal adenomas with severe dysplasia.The k-ras expression rate was significantly correlated with the positive expression rate of cyclin D1 and c-myc, The c-myc expression rate was significantly correlated with the positive expression rate of cyclinD1.Conclusion: Abnormality of many genes involved in ras signaling pathway of k-ras,c-myc and cyclinD1 may be involved in the molecular mechanism of colorectal carcinoma and Colorectal adenoma,Over-expression of k-ras in the colorectal carcinoma may activate the expression of oncogene cyclin D1 and c-myc. The overexpression of k-ras,c-myc and cyclinD1 may coexist in the development of colorectal carcinoma and Colorectal adenoma. which plays a pivotal role in the carcinogenesis and progression of colorectal carcinoma and Colorectal adenoma . The positive rate and expression quantity of k-ras,c-myc and cyclinD1 in Colorectal adenoma were higher than normal Colorectal tissue (P<0.05).Colorectal tubular adenoma has been changed in the gene level. The positive rate and expression quantity of k-ras,c-myc and cyclinD1 in Malignant transformation of colorectal adenoma organizations were higher than Colorectal adenoma.Expression of k-ras,c-myc and cyclinD1 play a role in the early stages of Malignant transformation of colorectal mucosa . Over-expression of k-ras,c-myc and cyclinD1 may be associated with the occurrence and development of colorectal cancer and colorectal adenocarcinoma,and which may be used as an important biological marker and one of the signs of disease Assessment.