Expression Of MiR-152,miR-377 And MiR-411 In The Human Placentas Of Patients With Preeclampsia

MicroRNAs (miRNAs) are a newly found 19-25nt single standed noncoding RNAs, and were distributed widely in Eukaryotes from C. elegans to human beings. MiRNAs have a characteristic of highly evolutionarily conservation and exihibit tissue-specific or developmental-stage-specific expression. Most of their genes are not randomly distributed but often closely clustered on the genome . MiRNAs act as a gene regulation molecular and function through the regulation of target genes by imperfect or perfect base-pairing with their target mRNAs, then either lead to the degradation of the target transcript (near perfect complementarity ) , or the inhibition of the protein translation Through above mechnisim miRNAs play a important role in gene regulation network and play critical roles in many biological processes including the regulation of development,cell proliferation or differentiation,apoptosis and cancer formation . Recent years many studys reported that miRNAs and genes coding for the enzymes involved in miRNA biogenesis were expressed and have influence on the development and pathogenesis of many disease.But presently there is no report about expression and function of miRNA in preeclampsia.In this study, we intended to investigate the expression of miRNA in preeclampsia to find the evidence of miRNA acting on development and pathogenesis of preeclampsia.AIM:Investigate the expression of miRNA in the human placentas of patients with preeclampsia to further elucidate the functions of miRNA on preeclampsia development.METHODS:Placentas were obtained from patients with preeclampsia and the nomal parturients; Looped miRNA specific RT primers were used to reverseiy transcribe the miRNA, miRNA were then amplified by SYBR Green real-time PCR using miRNA specific PCR primers.RESULTS:Differential expression between preeclampsia and the control group was found. The expression of miR-152 in the human placentas of patients with preeclampsia is up, miR-377 and miR-411 are depressed .CONCLUSION:The present study suggests that the specific miRNA are distinctive expression in the human placentas of patients with preeclampsia, preeclampsia development could be controlled by the specific miRNA.