Expression And Intracellular Localization Of MSP58 In Human Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is one of the most common malignancies in China. About 110 thousands of pepole die of HCC each year, accounting for 45% of the HCC related deaths in the world. Therefore, identifying key molecules that participate in the mechanisms underlying the carcinogensis and development of HCC, and exploiting these molecules to reverse the malignant biological behavors of HCC have been paid more and more attentions nowadays. MSP58 , identified as a downstream molecule of NDRG2 in our previous study, can facilitate the proliferation of Human Glioma cells . Other studies showed that MSP58 is a cell cycle-dependent transcription factor. MSP58 can promote the cells colony forming ability and malignant phenotype of anchorage-independent growth, and MSP58 is named after a protooncogene that interacts with the important molecules relating to tumorigenesis and tumor hyperplasia. It means that the MSP58 is a very important oncogene for tumor growth, and this gene maybe become a therapeutic target in many tumor. Up to date, although the expression of MSP58 was well defined in some other of tumors, the expression of MSP58 in HCC has not been reported. In this study, the expression of MSP58 mRNA and protein were detected in HHCC, HepG2 cell lines and tissue of HCC to show the regular pattern of MSP58 expression.Part I Expression of MSP58 in HHCC,HepG2 cell linesObjective To identify the expression of MSP58 mRNA and protein in HHCC,HepG2 cell lines. Methods RT-PCR and Western blot techniques were employed to examine the expressed extent of MSP58 messionger RNA and protein in HHCC,HepG2 cell lines. Results There were not significant difference between the two tumor cell lines(P >0.05). Conclusion There is not significant difference between the expressions of MSP58 in the HHCC,HepG2 cell lines.Partâ…¡Expression and localization of MSP58 in the cell of HCC tissueObjective To identify the expression of MSP58 mRNA and protein and the intracellular localization of MSP58 protein in the HCC tissue. Methods 100 HCC tissue samples and 30 normal liver tissue samples were randomly collected in this experiment. The tissue samples were divided into control group (normal liver tissue,n=30) and other three groups in different pathological grades (â… ,n=32;â…¡,n=53;â…¢,n=15). Immnohistochemical technique was employed to examine the expression extent and the localization of MSP58 protein in all the samples. Results Expressed extents of MSP58 protein in the samples of control group were not found except 7 samples in very low expression. The expression of MSP58 of the samples in the other three groups in different pathological grades were higher than that of control group(P <0.05), and there were also significant difference between the four tumor sample groups in a level dependence manner(P <0.05). The main zone of localization of MSP58 protein was cytoplasm in HCC cell. Conclusion There is significant expression of MSP58 in protein level in the tissue of HCC compared with normal liver tissue; expressed extent of MSP58 is going up in a level dependence manner; the main zone of localization of MSP58 protein is cytoplasm in HCC cell.