Comparing The De-epithelialization Effects Of Intestine By Dehydrated Alcohol With By Silver Nitrate: An Experimental Study In Rabbits

Objective: To observe and compare the de-epithelialization effects of intestine treated with dehydrated alcohol or Silver Nitrate on rabbits model.Methods: Twenty-five rabbits were randomly divided into five groups, five in each group. All rabbits were anesthetized before operation. A 20cm ileum segment was isolated with intact vessel. The ileum segments were treated in each group respectively with:(A)dehydrated alcohol for 8min,(B)dehydrated alcohol for 15min,(C)6%Silver Nitrate solution for 8min(D)3%Silver Nitrate solution for 15min(E)0.9% Sodium Chloride for 8min.At the end of four weeks, we observed the absorptive function of the segments through Glucose-absorption test and also observed the histological changes.Results: Both dehydrated alcohol and Silver Nitrate can effectively de-epithelialize intestinal segments. Comparing with the control group, the mucosa from four treatment groups were atrophied (significance<0.01).The Glucose-absorption test demonstrated the absorptive function of the ileum segments in treated groups was decreased(significance<0.05).The group A and group C has a better result, mucosa from the two groups has fewer absorptive and secretive function, but when comparing the histological changes between them, it shows no significant differences.Conclusion: The de-epithelialization effect is obvious either in group A(by dehydrated alchol for 8min) or in group C(by 6%Silver Nitrate solution for 8min) , the absorptive and secretive function are effectively depressed. We have found no histological differences between group A and C.