Expression And Significance Of Neuropeptide Y Adrenomedullin In Placentas Of Pre-eclampsia

Objective: Hypertensive disorder complicating pregnancy is a special disease in the trmester of pregnancy, affecting approximately 9.4% of women. HDCP has a strong impact on maternal and fetal health.It is the major cause of maternal and fetal morbidity and mortality.But the cause of the disease is not clear yet.ADM is a sort of bioactive peptide which can expand blood vessel,so far,it is one of the strongest vasodilators.In the trimester of pregnancy,matrix and placenta can secretion ADM.ADM can expand patient's placenta blood vessel,lower placenta circulation resistance,assuring enough placenta blood perfusion.ADM can regulate maternal blood stationariness either by circulation hermones or by itself and/or paracrine factor regulating partial blood tension and hermone secretion of fetal placenta.NPY is a bioactive peptide discovered and separated from pig brain and is a sort of bioactive peptide having very strong contracting blood vessel function,having reinforcing other endogene contracting blood vessel substance effects,inhibitioning the expanding blood vessel substance effects.The human's placenta can generate and release NPY and there is the NPY receptor in placenta,cocxisting in placenta with other hormone such as GnIH.This topic plans to investigate the expression of ADM and NPY in placenta of woman with preeclampsia and normal pregnancy, and analyse the relations with HDCP, and to make a further more study of their effect in HDCPand clinical significantion.Method: Placental samples were obtained in 30 minutes after delivery from 35 normal pregnancy women and 37 patients with mild preeclamptic pregnancies, 34 with severe preeclampsia. The villi were taken from them. All placentas were obtained from women undergoing spontaneous labor when they took on caesarean surgery. All tissues were routinely fixed in 10 percent formalin and embedded in paraffin. Four-μm-thick tissue sections of all samples were stained by immuno- histochemical analysis. Statistical analyses were performed with the use of the SPSS 13.0. Kruskal-Wallis H test and spearman rank correlation were used to analyze the data,α=0.05 was considered statistically significant.Result: 1The three groups were matched in mean age, gestational weeks, parity, body mass index and placental weight. 2 The comparison of ADM's location and positive expression rate in pregnant woman placenta tissue: The location of ADM in eclampsism placental tissue is as in normal late pregnant women. ADM was localized to syncytial trophoblastic cell, cytotrophoblast cell and vascular endothelial cell. The concentrations of ADM on normal placenta cells were high, positive rate was 45.71% and that of mild preeclampsia were higher, positive rate was 64.86%, severe preeclampsia had highest expression, positive rate was 91.18%(showed by Fig.1, 3-5, table 2). The difference between the normal placenta and preeclamptic placenta was statistically significant (P<0.05); the difference between the normal placenta and mild preeclamptic placenta was statistically significant (P<0.05); the difference between the normal placenta and severe preeclamptic placenta was statistically significant (P<0.01); the experiments found significant differences in ADM by severe and mild preeclampsia placenta (P<0.05). 3 The comparison of NPY's location and positive expression rate in pregnant woman placenta tissue: NPY was localized to trophocyte, vascular endothelial cell and trophonerna stroma. The concentrations of NPY on normal placenta cells were high, positive rate was 31.43% and that of mild preeclampsia were higher, positive rate was 51.35%, severe preeclampsia had highest expression, positive rate was 79.41%(showed by Fig.2, 6-8, table 3). The difference between the normal placenta and preeclamptic placenta was statistically significant (P<0.05); the difference between the normal placenta and mild preeclamptic placenta was statistically significant (P<0.05); the difference between the normal placenta and severe preeclamptic placenta was statistically significant (P<0.05); the experiments found significant differences in NPY by severe and mild preeclampsia placenta (P<0.05). 4 Positive correllation was found between ADM and NPY expressions in normal groups (r=0.3462 p<0.05), in patients with mild and severe preeclampsia, ADM expression showed positive correlation with NPY expression (r=0.4328 p<0.05; r=0.7833 p<0.05).Conclusion: Normal pregnancy placental villus express ADM and NPY, ADM is secreted by synetiotrophoblast, cytotrophoblast cell and vascular endothelial cell. NPY is secreted by trophocyte, vascular and othelial cell and trophonerna stroma. The severity of patient's condition in eclampsism is close to expression of these two factors. Further, the expression of ADM and NPY in normal pregnancy and eclampsism placental tissue shows positive correlation. The placental tissue can generate ADM, NPY. ADM and NPY are participated in the pathology and physiology procedure of HDCP.