The Animal Model Of De Novo Scoliosis And Imageology Observation

De novo scoliosis is one of the severe degenerative spinal disease. Because of the complexity of clinical presentation, different kinds of treatment selection and imprecise clinical efficacy, it is one of the most disturbing diseases for spinal surgeons. The further study of the pathogenesis of De novo scoliosis has been the research focus. An animal model which is scientific, economical, short–cycle and practical, is very important for the clinical medicine and medical research.Use spinal needle to puncture the left side of the annulus fibrosus and inject 10% hypertonic saline. Attempt this method to set up the animal model of De novo scoliosis. Observe the changes of spine morphology and disc signal by CR and MRI, find out the difference before and after operation( 3 months).Objective:Attempt to set up a model of De novo scoliosis by intervention in intervertebral disc and paraspinal muscles, and observe the changes by CR and MRI; Based on the experimental data, preliminary discuss the mechanism of De novo scoliosis.Methods:18 New Zealand white rabbits were randomly divided into three groups: Control Group (6), Test Group One (6) that the annulus fibrosus was only dealed with by 7th puncture needle, Test Group Two (6) which were treated with puncture and injection the 10% hypertonic saline. Expose the left part of annulus fibrosus of L2/3, L3/4, L4/5 disc by using lumbar anterolateral approach. Holding the 7th puncture needle with needle forceps, puncture at the point which is 1mm from the inner side of the left transverse process. Make sure that puncture direction parallel with the end-plate to prevent endplate injury. Control puncture depth at 4mm for the Test Group One. In Test Group Two, it is based on the operation mentioned above, and pull out the stylet from the puncture needle, pump a little nucleus pulposus, then inject 10% hypertonic saline. Distinguish the changes of spine mophology and disc signal after operation( 3 month) from those before the operation by CR and MRI.Results:CR examination before the operation exclude congenital scoliosis; There are four rabbits which show visual scoliosis by CR 3 months after the operation and one with spinal instability; Comparing the MRI result of the three groups shows that the disc signal of the puncture group and the puncture and injection group is diminished, the Test Group Two is more obvious than the Test Group One; Measure the intervertebral space height of the degenerative disc and control disc and analyse the data before and after operation, find out they are in no statistical sense after compared left sides with right sides. Conclusions:The method (Puncture the annulus fibrosus and inject 10% hypertonic saline ) can cause degeneration of disc in 3 months. There is one with spinal instability, it shows the significance to continue observing the changes of animal model. The loss of vertebral height, the scar from the muscle injury of operation, the abnormal posture caused by surgical stimulation may lead to the scoliosis.