Expressions Of Laminin In Serum Of Patients With Intractable Epilepsy

OBJECTIVE: To study the difference in serum expression of laminin in patients with intractable epilepsy, and further investigate and provide evidence for pathogenesis of intractable epilepsy.METHOD: The expressions of laminin in serum of 30 patients with intractable epilepsy, 46 patients with non- intractable epilepsy and 30 normal persons were measured by ELISA and western blot.RESULTS: ELISA showed that the expressions of laminin were as follows: the intractable epilepsy group– 177.396±30.602; the non-intractable epilepsy group– 121.915±35.215; and the normal control group– 67.474±7.197. The expressions of serum laminin in the intractable and non-intractable epilepsy groups were higher than that of the normal control group, while the intractable epilepsy group was even higher than the non-intractable epilepsy group. In the results of western blot, the ratios of optical density between laminin andβ-actin were as follows: the intractable epilepsy group 0.871±0.032; the non-intractable epilepsy group 0.686±0.017; and the normal control group 0.385±0.024. The ratios of the intractable and the non-intractable epilepsy groups were higher than that of the normal control group, while the intractable epilepsy group showed even higher value than the non-intractable epilepsy group.CONCLUSION: Laminin significantly increases in patients with epilepsy and this is more profound in patients with intractable epilepsy.Laminin ,therefor- e,may participates in the developmental process of epilepsy, and maybe an important factor that induces the refractoriness of epilepsy.