Toll Like Receptor 9 And 10 In Pleural Effusion

Objective The aim of this study was to investigate the expression of the toll like receptor (TLR) 9 and 10 in pleural effusions(PEs) of various causes and to analyze the influential factors.Methods The pleural fluid and peripheral blood concentration of TLR 9, 10 were measured using ELISA from 16 patients with infectious pleural effusion, 32 patients with tuberculosis pleural effusion, 33 patients with neoplastic pleural effusion, and 12 patients with transudate by. Meanwhile, we also detected the concentrations of some biochemical indicators such as lactate dehydrogenase (LDH), C-reactive protein (CRP), glucose (GLU), protein (PRO) in PE and serum. Finally, the relationships between TLRs and biochemical indicator were analyzed.Results (1) The concentrations of PE TLR9 were significantly different among all four groups (P<0.01). The concentrations of TLR9 in bacterial group [25.36 (28.50) ng/ml] were significantly higher than those in malignant group[10.63 (17.76) ng/ml] (p<0.05). The concentrations of TLR9 in malignant group were significantly higher than transudative group[2.20(9.19) ng/ml] and tuberculosis group[2.35 (5.84) ng/ml] (p<0.01), but there were no difference in the concentrations of PE TLR9 in transudative group and tuberculosis group. The serum TLR9 concentrations were significantly different among the four groups (p<0.01). The concentrations of serum TLR9 in tuberculosis group[0.28(0.32) ng/ml] were lower than those in transdative group[0.75(2.93) ng/ml], malignant group[0.46(0.47) ng/m] (both p<0.05). There were no difference in the concentrations of serum TLR9 in other groups.TLR9 concentration were much higher in PEs than in serum with four groups,respectively (p<0.01). (2) The PE TLR10 concentrations were significantly higher in bacterial group[2.6(1.86)ng/ml] than those in malignant group [1.82(0.58) ng/ml](p<0.05), but there were no difference in the concentrations of PE TLR9 in other groups. The serum TLR10 concentrations were significantly different in all groups(p<0.01).The concentration of serum TLR10 in transudative group were highest(4.09±1.84ng/ml)(p<0.05). The concentration of serum TLR10 in malignant group [2.43(2.12) ng/ml]were significantly higher than tuberculosis group[1.62(1.60 ng/ml)] (P<0.05). There were no significant difference in bacterial group and tuberculosis group or malignant group. The concentrations of serum TLR10 in transudative group were significantly higher than those of PE TLR10( p<0.05). In other groups, TLR10 in PE were no significantly difference with in serum (P>0.05). (4). The PE TLR9 levels were positive correlated with the serum TLR9 levels in transudative group(n=12,r=0.832, p<0.001); the PE TLR9, 10 levels of malignant group were also positive correlated with PE LDH levels(n=33,r=0.609, p<0.01, n=33,r=0.49, p<0.01, respectively). Besides, the PE TLR10 levels were positive correlated with the levels of PE CRP(n=33,r=0.516, p<0.01). The serum TLR10 of malignant group were positive correlated with the serum LDH in tuberculosis group(n=32,r=0.486, p<0.01) and malignant group(n=33,r=0.399, p<0.05). The serum TLR9 were positive correlated with the serum CRP in bacterial group(n=16,r=0.621, p<0.05) and malignant group(n=33,r=0.387, p<0.05).Conclusions: (1)The concentrations of TLR9 in bacterial group are significantly higher than non-bacterial group. (2)The detection of PE TLR 10 are not helpful to differentiate bacterial PE from other else.(3) TLR9 and TLR10 may take part in the mechanisms of malignant PE in different ways, respectively.