Effects Of HDL Preconditioning On Ischemia-reperfusion Lung Injury In Rat

Studies have showed that lung is one of the most preferred target organs of ischemia-reperfusion injury.This injure including lung edema,hypoxemia and the damage in gas exchanging.To investigate the effects of ischemia preconditioning in ischemia-reperfusion lung injury,24 healthy female Sprague-Dawley rats were anesthetized by sodium pentobarbital.Building experimental model of rat lung orthotopic allograft transplantation.HDL/ Ischemic-preconditioning group(H/IPC)was given HDL before ischemia-reperfusion injury.The concentration of MDA and the activity of SOD and MPO,wet to dry weight ratio(W/D) in the lung tissue were determined respectively in different groups.Pathologic changes of the lung tissue in different groups was also observed.Results1.Compared with IR group:H/IPC could rise PaO₂ and low PaCO₂, significantly improve the exchange function.At the same time,it not only markedly reduce the ratio of W/D,but also significantly reduce the inflammatory cells in the lung tissue.2.Compared with SO group:The concentration of MDA and activity of MPO in the lung tissue was elevated(P＜0.01),while the activity of SOD was significantly reduced(P＜0.01).The H/IPC group shows indifference against SO group.3.Under light microscope:The lung tissues structure was clear and alveolar wall was thin in SO group,while alveolus interval widen and edema obvious in IR group.H/IPC group was significantly lighter than IR group and indifference against SO group.ConclusionH/IPC can be able to relieve the increase in permeability of lung capillary and to inhibit the excessive product of oxygen-derived free radicals,lessen the infiltration of PMN in the ischemic lung tissue at the same time.It may play a protective role on lung reperfusion injury...