The Infiltration Of MC,MΦ And Expression Of TGF-β₁ In IgA Nephropathy

objective To observe the infiltration of Mast cells (MC), macrophages (MΦ) and the expression of transforming growth factor-β1 (TGF-β1) in IgA nephropathy (IgAN). Investigate their relationship between the infiltration of MC, MΦand the expression of TGF-β1 and IgA nephropathy in tubulointerstitial lesions(TIL) .Methods The 49 renal biopsy specimens were made by renal biopsy from IgA nephropathy patients in Nephrology Department of the second hospital of Shanxi medical university from April, 2007 to April, 2008. All the IgA nephropathy cases were confirmed by light microscope, immunofluorescence and transmission electron microscope. The tubulointerstitial lesions of IgA nephropathy were divided into 0,ⅠandⅡrespectively according to three areas which including renal tubuli lesions, tubulointerstitial inflammation and renal interstitial fibrosis. The 9 cases normal renal were the tissue far from nephronophthisis or tumor of kidney over 2cm. The expression of Tryptase, CD68 and TGF-β1 were detected by immunohistochemistry in nephridial tissue . The relation between MC(Try+ cells ), MΦ(CD68+ cells) and TGF-β1 and the tubulointerstitial lesions of IgA nephropathy, and the associativity between they and serum creatinine ,24h urine protein volume were analyzed.Results 1 The infiltration of MC, MΦand the expression of TGF-β1 were found occasionally in normal renal tissue. The infiltration of MC, MΦand the expression of TGF-β1 were significantly enhanced in IgA nephropathy (the positive rate of mast cell was calculated to be 42.86% (21/49), the positive rate of macrophage was calculated to be 40.82% (20/49), the positive rate of transforming growth factor-β1 was calculated to be 51.02% (25/49)).2 The degree of renal tubuli lesions and tubulointerstitial inflammation IgA nephropathy were not significant between Tryptase positive groups and negative groups(P>0.05), the degree of renal interstitial fibrosis, the difference was significant (χ2 = 10.1577, P <0.05); the degree of renal tubuli lesions and renal interstitial fibrosis were significant between CD68 positive groups and negative groups(χ2 = 7.1687, 8.3300, P <0.05), the degree of tubulointerstitial inflammation were not significant (P>0.05); the TIL were not significant between TGF-β1 positive groups and negative groups (P>0.05). The degree of renal interstitial fibrosis were serious gradually in accordance with the numbers of the infiltrated MC in IgA nephropathy. The numbers of the infiltrated MC <10 / section were 16 cases in Try+ groups, there is 1 case which was was moderate (Ⅱlevel) interstitial fibrosis , the numbers of MC >10/ section were 5 cases, all was moderate (Ⅱlevel) interstitial fibrosis, the difference was significant (χ~2 = 16.4052, P <0.005).3 The numbers of the infiltrated Try~+ cells, CD68~+cells in tubuli and interstitium of IgAN was significant between TGF-β1 positive group and negative group(P<0.05).4 The numbers of Mast cell positively correlated with serum creatinine (r = 0.65, P <0.001), no correlated with 24h urine protein volume (r = 0.18, P> 0.05); quan- tity of macrophages positively correlated with serum creatinine and 24h urine protein volume (r = 0.59, 0.48, P <0.01) ; transforming growth factor-β1 positively correlated with serum creatinine and 24h urine protein volume (r = 0.42, 0.35, P <0.05).Conclusions?1 Mast cells, macrophages may be the important participants of renal interstitial fibrosis of the IgA nephropathy;2 transforming growth factor-β1 may be the important chemokine of mast cells and macrophages.