| Objective To observe the effect of anti-CD40L mAb on inducing allogeneic immunologic tolerance ; to character the role of CD40/CD40L pathway in transplant immune response .Methods Wistar rats were intravenously injected STZ60mg/kg to induce Type-1 diabetes, Type-1 diabetes rats randomly were divided into 3 groups, group A diabetes rat , group B : Islet transplantation + anti-CD40LmAb; Group C : Islet transplantation without mAb treatment. To inject anti-CD40L monoclonal antibody (20ug/only), islet cells were and isolated and purified from SD rats. Islet cells were obtained at (3.49±0.23)×105IEQ/kg . Islet transplantation was performed at the density of 12000 IEQ/kg by the way of portal vein injection . Recipient's blood glucose was monitored everyday and body weight was recorded weekly after transplantation. IL-2 was examined on 7d, 15d and 22d.Result Blood glucose went back to normal level on d3 after transplantation in Group B (8.15±1.40)mmol/L and Group C (7.42±2.55) mmol/L. The normal glucose can be kept for 18.12±4.12 days in Group B and 8.00±1.30 days in Group C. Regarding to the body weight, In group A: body weight continually dropped significantly after transplantation. In Group B and Group C , body weights increased with the recovery of plasma glucose .but dropped as soon as the rejection occurred. The expression of IL-2 was inhibited in Group B (106.0±4.9) pg/ml,(64.2±9.3) pg/ml and Group C (494.5±11.5) pg/ml,(470.4±8.0)pg/ml on the d7 and d15 respectively(P<0.001). No significant difference was displayed between Group B and Group C after d22 of post-transplantation.Conclusion Administration of anti-CD40LmAb can significantly prolong the survival time of allogeneic islet grafts; The blockage of CD40/CD40L co-stimulating pathway is a potential protocol for inducing allogeneic transplant tolerance .
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