Expression Of Dsg1 MRNA In Lesional Psoriatic Epidermis.

Background:Psoriasis is a chronic, recurrent inflammatory skin disease characterized by epidermal hyperproliferation. The underlying pathogenic mechanism is still not completely clarified. Self-active T cell-mediated immune response is thoughted to be the main pathological mechanism of psoriasis.However,in recent years numerous studies have demonstrated that apoptosis plays a key role in the pathogenesis of psoriasis and that aberrant expression of many apoptosis-related molecules is related to be abnormal apoptosis of psoriatic keratinocytes.Objective:To explore the action of desmoglein 1(Dsg1)in apoptosis of lesional psoriatic keratinocytes by examining the expression of dsg1 in the psoriatic and the normal epidermis.Methods:A total of 34 skin samples (Biopsies of 17 patients with psoriasis vulgaris and 17 normal skin) were treated by dispase for separating intact epidermis from the dermis.Then the expressions of Dsg1 and caspase-3 in the epidemis were examined by RT-PCR.Results:The psoriatic epidermis showed higher expression of dsg1 mRNA when compared to normal epidemis (0.74±0.12～0.54±0.10) (P < 0.05). There was no significant difference at caspase-3 mRNA level between the psoriatic and the normal epidermis(0.36±0.08～0.39±0.08) (P > 0.05)Conclusion:The results of the study suggested that abnomal apoptosis process of psoriatic epidermal keratinocytes might be related to the aberrant expression of Dsg1.