The Inhibitive Effect Of White Arsenic On Cervical Cancer Transplanted In Nude Mice And Its Influence On The Expression Of Bcl-2 Protein

Purpose1.By transplanting the Hela cell to the nude mice,we study the tumor suppressive effect of white arsenic to cervical cancer and explore the side effects and toxicities of them to the mice.2.To investigate the relation between the apoptosis and the protein expression of Bcl-2.Methods1.Hela cells were cultured at 37℃in a 5 mL/L CO₂ incubator.Thirty mice received subcutaneous injection with cell suspension containing 2×10⁷ cells.When tumors of about 0.5cm diameter were detected,drug administration was started.The animals were randomly divided into three groups including model group,carboplatingroup and white arsenic group. There are 10 animals in each group.2.After 15d of treatment,the mice were sacrificed and the tumor masses were removed.After the weight of tumor masses was measured,some were fixed in 10%paraformaldehyde,and some were frozen.The tissues of liver,kindy, spleen,stomach,large intestine and small intestine were stained with HE and analyzed for the side effects and toxicities of the drugs.3.Bcl-2 protein were detected with Immunohistochemical SP method.Results1.The weights of the tumors in carboplatin group and white arsenic group are more than in the model group(P＜0.05).In the all treatment groups,there was no differences in weights of the tumors(P＞0.05).The suppressive rates to tumor in carboplatin group and white arsenic group was 34.3%,35.8% respectively.These suggest that white arsenic can inhibit the proliferation of cervical cancer,and white arsenic has no toxicity to liver,lung,kidney,spleen,stomach,large intestine or small intestine.2.The expressions of Bcl-2 protein in the treatment groups were much lower than in the model group(P＜0.05).Conclusion1.As the carboplatin,white arsenic can inhibit the proliferation of cervical cancer.And white arsenic has no toxicity to liver,lung,kidney,spleen, stomach,large intestine or small intestine.2.White arsenic decease protein expression of Bcl-2.Down-regulating of Bcl-2 may play an important role in the apoptosis in cervical cancer cells.