The Research Of Clinical Correlation For BCG On RSV Infection Children

Object:To observe the relationship between BCG vaccination and RSV infection in children with clinical conditions,hospitalization days,lymphocyte subsets in the acute phase,the concentration of IL-4(CD8+) and IFN-γ(CD8+) in the acute infection phase, prognosis and atopic disposition.To discuss the mechanism of BCG vaccination on RSV infection.Method:According to the situation of BCG vaccination,tuberculin test(PPD skin test) results,personal history of allergy(such as eczema) and family history of allergy(such as asthma,allergic rhinitis,urticaria,etc.),we divide the 64 cases with RSV infection into: group A(BCG immunization positive group):children have been BCG vaccinated and have positive result of PPD skin test,a total of 22 cases;group A1:children without personal or family history of allergy in group A,a total of 11 cases;group A2:children with personal or family history of allergy in group A,a total of 11 cases;group B(BCG immunization negative group):children haven't been BCG vaccinated or have been BCG vaccinated but have negative result of PPD skin test,a total of 42 cases;group B1:children without personal or family history of allergy in group B,a total of 19 cases;group B2:children with personal or family history of allergy in group B,a total of 23 cases.The RSV was examined by indirect immunofluorescence;the lymphocyte subsets were analyzed by flow cytometry;the concentration of IL-4(CD8+) and IFN-γ(CD8+) was assayed by ELISA. Follow up analysis about one year.Results:1.1 cases with severe wheezing in group A are significantly less than that in group B(P＜0.05).There is no statistical difference between group A1 and A2,group B1 and B2;1.2 cases with severe pulmonary signs in group A are significantly less than that in group B(P＜0.05).There is no statistical difference between group A1 and A2,groupB1 and B2;1.3 hospitalization days in group A are significantly less than that in group B(P＜0.05). There is no statistical difference between group A1 and A2,group B1 and B2.2.1 the percentage of CD3+ is significantly lower than normal in group A,A1,A2,B, B1,B2(P＜0.05).The percentage of CD3+ in group Alis significantly higher than that in group A2(P＜0.05).There is no statistical difference between group A and B,groupB1 and B2;2.2 CD4+/CD8+ is significantly lower than normal in group A,A1,A2,B1, B2(P＜0.05),but is significantly higher than normal in group B(P＜0.05).There is no statistical difference between group A and B,groupA1 and A2,goup B1 and B2;2.3 the percentage of CD3-CD(16+56)+ is significantly lower than normal in group A, A1,A2,B,B1,B2(P＜0.05).There is no statistical difference between group A and B, groupA1 and A2,goup B1 and B2;2.4 the percentage of CD19+CD23+ is significantly higher than normal in group A, A1,,B,B1,B2(P＜0.05).There is no statistical difference between group A2 and normal(P＞0.05).The percentage of CD19+CD23+ in group A is significantly lower than that in group B(P＜0.05).There is no statistical difference between group A1 and A2,group B1 and B2;2.5 the percentage of CD3+CD25+ is significantly lower than normal in group A,A1, A2,B,B1,B2(P＜0.05).There is no statistical difference between group A and B,groupA1 and A2,group B1 and B2.3.1 there is no statistical difference in the concentration of IL-4(CD8+) between group A and B.The concentration of IL-4(CD8+) in group A1 is significantly lower than that in group A2(P＜0.05).The concentration of IL-4(CD8+) in group B1 is significantly lower than that in group B2(P＜0.05);3.2 there is no statistical difference in the concentration of IFN-γ(CD8+) between group A and B.The concentration of IFN-γ(CD8+) in group A1 is significantly higher than that in group A2(P＜0.05).The concentration of IFN-γ(CD8+) in group B1 is significantly higher than that in group B2(P＜0.05).4.1 cases without wheezing re-attack within one year in group A is significantly less than that in group B(P＜0.05).There is no statistical difference between group A1 and A2. Cases without wheezing re-attack within one year in group B1 is significantly less than that in group B2(P＜0.05).4.2 there is no statistical difference in cases with wheezing re-attacks more than 2 times within one year between group A and B,group A1 and A2,group B1 and B2.Conclusion:1 BCG vaccination can improve the clinical condition,reduce the number of days in hospital on children with RSV infection,and is not influenced by atopic disposition.2 the percentage of CD3+,CD3-CD(16+56)+ and CD3+CD25+ are significantly less than normal.The percentage of CD19+CD23+ is significantly higher than normal.BCG vaccination can reduce CD4+/CD8+ and the percentage of CD19+CD23+,and is not influenced by atopic disposition.3 there is no significantly relationship between BCG vaccination and the concentration of IL-4(CD8+) or IFN-γ(CD8+).The concentration of IL-4(CD8+) in cases without personal or family history of allergy is significantly lower than cases with personal or family history.The concentration of IFN-γ(CD8+) in cases without personal or family history of allergy is significantly higher than cases with personal or family history.It is rational to use IFN-γfor children with personal or family history of allergy.4 BCG vaccination can reduce the risk of wheezing re-attack,and has not distinct relation with atopic disposition.