The Research Of Mechanism Of Propofol Inhibition Hippocamp Neurons Against Apoptosis Through Inducing HO-1 Protein Expression

Objective:To investigate the neuroprotective elects of propofol on oxygen-glucose deprivation injury to primary culture rats hippocamal neurons.Methods:Newbom(24～48h)Sprague-Dawlay(SD)rats hippocampus neurons were cultured 7 days.On the 7th day the cultured hippocampus neurons were randomly divided into 5 group(n=36):the normally cultured cell group(Group C1),the normally cultured cell+50μmol/L propofol group(Group C2),the oxygen-glucose deprivation(OGD)group(Group D),OGD+50μmol/L propofol group(Group P), OGD+50μmol/L propofol+10μmol/L ZnppIX(Group Z).Group C1 were normally incubated another 25 h.Group C2 were incubated another 24 hafer hippocampus neurons were anesthesized with 50μmol/L propofol for 1h.Group D were normally incubated another 24 hours afer hippocampus neurons were carried out OGD 1 h.Group P were normally incubated another 24 hours after hippocampus neurons were carried out OGD and meanwhile were anesthesized with 50μmol/L propofol 1 h. Group Z cells were incubated normally for another 24 hours after hippocampus neurons were carried out OGD 1 hour and meanwhile were added 50μmol/L propofol and 10μmol/L ZnppIX.After normal culture 24 h,apoptosis rate was detected by Hoechst33342 staining technique and fluorescence microscopy.HO-1 protein and Bcl-2 protein expression was detected by immunocytochemical technique.Results:Compared with group C1,the expression of HO-1 and Bcl-2 were both increased(p＜0.05) and apoptosis rates were not change(p＞0.05) in group C2 Compared with group D,the expression of HO-1 and Bcl-2 were both increased(p＜ 0.05) and apoptosis rates were decreased(p＜0.05) in group P.Compared with group P,the expression of HO-1 and Bcl-2 were both decreased(p＜0.05) and apoptosis rates were increased(p＜0.05) in group Z.Conclusion:Propofol inducing HO-1 expression protects neuron against oxygen-glucose deprivation injury through up-regulating bcl-2.