Research On Impact Of YunnanBaiyao On Platelet-Rich Plasma For Proliferation And Differentiation Of Bone Marrow Stromal Cells

[Objective]To study the direct impact of YunnanBaiyao on platelet-rich plasma (PRP) for proliferation and differentiation of the bone marrow stromal cells of the rabbits;to study the impact of YunnanBaiyao which were taken though oral on PRP for proliferation and differentiation of the bone marrow stromal cells of the rabbits.[Methods]The bone marrow stromal cells were isolated from the 4-month-old New Zealand rabbits as samples by using the adherent method.The Microscopy was used to study the cells's shape,and the flow cytometer was used to identify the cells.Observed the proliferation status of the 1^st,2^nd,3^rd,4^th,5^th,6^th,7^th,and 8^th generation cells,and drew the growth curve of the cells.Fed the rabbit with 100mg/Kg YunnanBaiyao once per day for 7 days.Obtain the blood samples of the rabbit on the 3^rd,5^th and 7^th dav respectively.Obtained one pure PRP sample from the rabbit that has not been fed with YunnanBaiyao and one PRP sample from the rabbit that has been fed with YunnanBaiyao by using the Landesberg method.Then,check the jinfluence of YunnanBaiyao on the PRP for the proliferation and differentiation of rBMSCs by the MTT and enzyme kinetics methods.[Results]Comparison between the YunnanBaiyao in vitro direct intervention groups and the control group:The difference between 60μg/m(0.53±0.03) and 65ug/m(0.56±0.051 sample goups on proliferation of rBMSCs(P＜0.05) is distinct.Sample goup 65μg/ml(1.21±0.08).70μg/ml(1.28±0.05),and 75μg / ml(1.24±0.06) proves that YunnanBaiyao can enchance the ALP activity(P＜0.05) of the rBMSCs.Comparison between the YunnanBaiyao oral intervention group and the control group: For the sample groups that have taken YunnanBaiyao for 5 days(0.30±0.02) and 7days(0.34±0.08),the one that has taken YunnanBaiyao for 7 days(0.88±0.06)proves that YunnanBaiyao can better enhance the ALP activity(P＜0.05) of the rBMSCs.[Conclusion]PRP,activated by YunnanBaiyao,can promote the proliferation and differentiation of rBMSCs.The 60μg / ml and 65μg / ml sample group and the sample group that has taken YunnanBaiyao for 5 davs and 7 days show the best effect on proliferation of rBMSCs;and the sample group 65μg/ml,70μg / ml,and 75μg / ml that have taken YunnanBaiyao for 7days show the best effect on differentiation of rBMSCs.