The Study Of Basic Fibroblast Growth Factor In Hirschsprung's Disease

OBJECTIVE:To investigate the expression of basic fibroblast growth factor(bFGF)in stenosed segment, transitional segment and dilated segment of Hischsprung's disease, to discuss the effects of altering the intestinal wall microenvironment on enteric nervous system maturation and to analyze the aetiology.METHODS:39 tissues of intestinal wall in stenosed segment, transitional segment and expansionary segment was collected from 2003-2009 from the Pediatrics surgery department at the first clinical hospital of Jilin university, aging from 2months to 5years, 30 were from male kids and the other 9 were from female kids, the 39 intestinal wall tissues were all evaluated by HE staining and immunohistochemistry (SP staining) of Hischsprung's disease without any other malformation. The correlation between the expression of bFGF and its clinicopathologic features was analyzed accordingly. The 39 intestinal wall tissues were cut in several series of 5um-thick onto the slides, all the slids were performed by HE staining or immunohistochemistry staining. The staining tissues were detected under the 20 times microscope, the brown staining parts were recognized as positive, the positive proportion under 10% were scaled 0 point, 11%-25% were scaled 1 point, 11%-25% were scaled 2 points, the positive proportion more than 50% were scaled 3 points. The staining intensity were scaled from 0-3 points according to the control ones, the final scales were classified as weak positive, medium positive and strong positive according to the scores of positive proportion plused staining intensity.RESULTS:Both the intramuscular nervous plexus of ganglionic segment and intermuscles were detected at expansionary segment according to the HE staining, and intramuscular nervous plexus were detected at stenosed and transitional segment without ganglionic cells. The brown products of positive expression of bFGF were abundant both the intramuscular nervous plexus and ganglionic segment were obviously decreased in the aganglionic colon. The expression degree and staining of aganglionic segment were all lower than that of ganglionic segments. There were 76.9% expansionary segmens were scored as stong expression of bFGF staining, 5.1% were scored as weak expression, among the control ones at stenosed and transitional segment, 2.6% were scored as strong expression and 74.4% were scored as weak expression, with significant statiscic.CONCLUSION:bFGF in the aganglionic colon were obviously decreased, it suggest that bFGF decrease could affect the differentiation, development, maturity and settlement of intestinal neural crest cell. Finalyty, these disorders, lead to congenital aganglion cells in bowel of patients with Hirschsprung,s disease. Altering the intestinal wall microenvironment may be one of the etiologic factors of HD.