| The relationship between smoking and health has being regarded for a long time. On the basis of former studies,this paper studies on cytotoxicity and mutagenicity of different mainstream cigarette smoke,the main experimental results are as follows:1.The results of MTT experiment showed that:the cell survival rate of adding the tobacco essence and flavor is less than no additive is added in the tobacco;the cell survival rate of adding 2# tobacco essence and flavor is more than adding 1# tobacco essence and flavor;the cell survival rate of adding tobacco flavor is more than adding tobacco essence;the cell survival rate of adding 1# tobacco essence is more than adding 2# tobacco essence;the cell survival rate of adding 2#tobacco flavor is more than adding 1# tobacco flavor;In the group adding monomer flavor,the cell survival rate of adding vanilla bean derivatives is the lowest,trimethylpyrazine is the highest;In the group adding single cut-tobacco from the same producing area and different grade,the cell survival rate of B2F from northern hunan is more than X2F from northern hunan, In the group adding single cut-tobacco from the same grade and different producing area,the cell survival rate of C3F from Brazil is the lowest,the C3F from Dali is the highest.2.The results of ROS(reactive oxygen species) experiment showed that:the oxidative damage of adding the tobacco essence and flavor is more than no additive is added in the tobacco;the oxidative damage of adding 1# tobacco essence and flavor is more than adding 2# tobacco essence and flavor;the oxidative damage of adding tobacco essence is more than adding tobacco flavor;the oxidative damage of adding 1# tobacco essence is more than adding 2# tobacco essence;the oxidative damage of adding 2# tobacco flavor is more than adding 1# tobacco flavor;In the group adding monomer flavor,the oxidative damage of adding vanilla bean derivatives is the maximum,trimethylpyrazine is the least;In the group adding single cut-tobacco from the same producing area and different grade,the oxidative damage of X2F from northern hunan is more than B2F from northern hunan,In the group adding single cut-tobacco from the same grade and different producing area,the oxidative damage of C3F from Brazil is the maximum,C3F from Dali is the least.3.Ames test showed that:the mutagenic capability of adding the tobacco essence and flavor is more than no additive is added in the tobacco;the mutagenic capability of adding 1# tobacco essence and flavor is more than adding 2# tobacco essence and flavor; the mutagenic capability of adding tobacco essence is more than adding tobacco flavor;In the group adding single cut-tobacco from the same producing area and different grade,the mutagenic capability of X2F from northern hunan is more than B2F from northern hunan;In the group adding single cut-tobacco from the same grade and different producing area,C3F from Brazil has strong mutagenicity;In the group adding monomer flavor,vanilla bean derivatives has strong mutagenicity,trimethylpyrazine has weak mutagenicity;in addition.the result of TA98 showed that 2# essence and 2# flavor have strong mutagenicity,but TA100 showed that 1# essence and 1# flavor have strong mutagenicity. |
