The Study Of The Change Of Th1/Th2 Cell In Re-treatment And Multiple-drug-resistant Tuberculosis

Tuberculosis is a disease caused by a facultative intracellular pathogen mycobacterium tuberculosis(MTB).Normal immunity can help human body erase mycobacterium tuberculosis in order to inhibit it develop into tuberculosis.Normal function of immune system depends on adherence among cells and cell-extracellular matrix,as well as functions among cell adhesion molecules and its mate.Immunity against MTB which is intercellular bacteria depends on specific cellular immunity which involves many cell factors.Specific cellular immunity plays an important role in fighting with tuberculosis.Most of tuberculosis patients can be cured by clinical treatment,but there are still some patients who have difficult in treating because of drug-resistance and other reasons.We have much pathogenic research in such patients, but few in their immunity.Objectives:To observe and value changes of immunity in retreatment and multiple-drug-resistance(MDR) TB patients.Thereby to help oncologist to adopt immune therapy,obtain maximum clinical efficacy,in tuberculosis.Methods:The patients' blood in accord with the selective standard is collected,and then IFN-r,,IL-2,IL-4及IL-10 is measured with enzyme-labeled immunosorbent assay(ELISA) CD3+,CD4+,CD8+,CD4+/CD8+ is measured with flow cytometry. Data were analyzed with T-test and rank sum test,The changes of the patients immune function can be evaluated.Results:a The statistic analytic results of cytokine IFN-r,,IL-2,IL-4 and IL-10 in healthy group,initial treatment group,retreatment group and MDR group show 1,The results of IFN-rin each group are not significant(P＞0.05).2,IL-2 between healthy group and initial treatment group are not significant(P＞0.05),the same result between retreatment group and MDR group.The result between healthy group and retreatment group;healthy group and MDR group;initial treatment group and retreatment group; initial treatment group and MDR group are significant(P＜0.01).3,IL-4 and IL-10 in each two group are significant(P＜0.01) except that it is not significant(P＞0.05) between retreatment group and MDR group.。The results of CD3+ in each group are not significant(P＞0.05).The results of CD4+,CD8+ and CD4+/CD8+ between healthy group and each TB group are significant(P＜0.01).CD8+ between initial treatment group and retreatment group,initial treatment group and MDR group are significant(P＜0.01).CD4+/CD8+ between initial treatment group and MDR group are significant(P＜0.01),CD4+,CD4+/CD8+ between initial treatment group and retreatment group are significant(P＜0.01),CD4+,CD8+,CD4+/CD8+ between retreatment group and MDR group are not significant(P＞0.05).Conclusion:The cellular immunity of TB patients decrease.It shows that the percent of CD4+T lymphocyte decrease while the percent of CD8+ T lymphocyte increase,CD4+/CD8+ decrease.Cytokines of IFN-r,and IL-2 which enhance the immunity against tuberculosis decrease while cytokines of IL-4 and IL-10 which inhibit the immunity against tuberculosis increase.Compared with initial treatment group,the immunity of retreatment group and MDR group are even worse,which can cause difficulty in treating.