A Study Of The Effects Of Vitamin E Succinate On Proliferation Of Human Lens Epithelial Cells

【Objective】To investigate the growth inhibition effects of vitamin E succinate (VES) on human lens epithelial cells cultured in vitro. And search for a new drug to inhibit posterior capsular opacification after cataract surgery.【Methods】The second or third generation of human lens epithelial cells were treated with different concentrations of VES (5μg/ml,10μg/ml,20μg/ml);culture media only (negative control group);culture media included 2‰absolute alcohol(solvent control group).1. MTT assay: Cells of different groups were cultured for 24h, 48h, 72h, 96h and measured the value of optical density to calculate growth inhibiting ratio respectively.2. Flow cytometer (FCM) analysis: The cell cycle of different groups were analyzed by Flow cytometer after treatment for 48h.【Result】1. MTT assay: There were no significant difference of proliferation inhibition between control groups and test groups treated with VES of 5μg/ml in 24h(P>0.05). But after 48h,72h and 96h, the lens epithelial cells were significantly inhibited (P<0.05).When the VES concentration up to 10~20μg/ml, there were significant difference of proliferation inhibition between control groups and test groups in 24h,48h,72h and 96h(P<0.05). VES could inhibit the proliferation of human lens epithelial cells in a dose-dependent and time-dependent manner.2. FCM analysis: There were significant difference of the cell cycle between negative control group and test groups in 48h (P<0.05). Showed that in test groups the rate of the G0/G1-phase cells increased, but the rate of the S+G2/M-phase cells decreased. VES could block the cells in G0/G1 phase stage.【Conclusion】Vitamin E succinate can significantly inhibit the growth of human lens epithelial cells cultured in vitro and the effect is dependently related to time and dosage. It is suggested that VES may be helpful in treating posterior capsular opacification.