| OBJECTIVE To investigate the effects of beta-catenin antisense oligodeoxynucleotide (ASODN) combined with paclitaxel on proliferation and expression of beta-catenin mRNA in breast cancer MCF-7 cell lines.METHODS The MCF-7 cells were transfected with beta-catenin ASODN, SODN and blank in different concentration, and the apoptosis index was measured after 48 hours. The colony forming efficiency was observed after 10 days when the concentration was 10μmol/L. And then, the MCF-7 cells were were transfected with beta-catenin ASODN, SODN, blank and paclitaxel in various combination. Expression of beta-catenin mRNA was measured by Real-time quantitative PCR after 24 and 48 hours, and cell growth was measured by MTT method.RESULTS The apoptosis index of MCF-7 cells was slightly increased when the concentration of beta-catenin ASODN was raised. There were statistical differences among ASODN group and SODN group. The colony forming efficiency had the same differences. The inhibitory efficiency and expression of beta-catenin mRNA of beta-catenin ASODN combined with paclitaxel was higher than that without paclitaxel (P<0.05) , also than that ASODN alone (P<0.05) .CONCLUSIONS ASODN can significantly suppress the proliferation and induce apoptosis of MCF-7 cells. Combined with paclitaxel, ASODN significantly decreases survival and beta-catenin at mRNA level of MCF-7 cells. |
