Effects Of Recombinant Interleukin-18 On Systemic Candidiasis In Mice

[Objective] To explore the expression of X-chromosome-linked inhibitor of apoptosis protein (XIAP) and its effect on apoptosis induced by low-dose Adriamycin on the bladder cancer cell line T24.[Methods] The XIAP gene was transferred into bladder cancer cell line T24 by liposome and the positive clone was screened by G418. Cellular XIAP mRNA level was detected by RT-PCR. Low-dose Adriamycin induces the apoptosis of T24 cells. The invitro cellular growth activities were assayed by MTT colorimetry; the apoptosis rate was assayed by acridine orange/ethidium bromide (AO/EB) double staining.[Results] XIAP mRNA level in transfected T24 cells was significantly increased by 3.8 times. Treated with 6μg/ml and 60μg/ml Adriamycin, the growth rates of XIAP transfected T24 cells were increased( 11.60%±0.25)%-(16.51±0.87)%(P<0.05) and the apoptosis rate was decreased (10.1±0.2)%-(11.9±0.2%(P<0.05) compared with the control cells.[Conclusion] Overexpression of XIAP in T24 results in decreasing apoptosis induced by Adriamycin, which could decrease the chemotherapeutic sensitivity of T24 cells.