The Research Of Prism Dispersion Compensation And Speckle Quasi-Confocal In Microscopy

Microscope with optical sectioning capability is widely used in biomedical research. On the one hand, fast scanning technique in multi-photon fluorescence microscope is an effective way to detect fast events in bioscience; however, fast scanning device will induce the problem of spatial dispersion. On the other hand, speckle quasi-confocal microscope is an economic new way for optical sectioning imaging without requirement on detection velocity, and has the latency for commercial use. In this thesis two techniques mentioned above are researched.In this thesis laser scanning confocal microscope is studied. The key points for constructing the microscope are analyzed, including scanning device placement, error of scanning device, aperture placement, and relay system usage and so on. The optical modules are designed to realize the microscope, and the relationship of optical design and the quality of image is expatiated as a basis of the work followed.Second, the spatial dispersion compensation of multi-photon random access microscopy is discussed. Based on the dispersion theory and the invention of spatial dispersion compensation of 2-D acousto-optical deflectors with a single prism, the engineering design of the compensation module is invented to decrease the difficulty of the microscope construction process. Further more, a spatial dispersion compensation module is invented and patented.Third, speckle quasi-confocal microscopy is researched. A speckle quasi-confocal microscope is constructed and the model experiment is developed to testify the quasi-confocal quality. Based on the theory of speckle quasi-confocal microscope, the influence of endoscope on the discipline of speckle fluctuation is discussed, and analytical expression is derived to describe the relationship between optical structure of endoscope and the axial resolution of endoscope-based speckle quasi-confocal system. The theory and experimental result show identically that endoscope-based speckle quasi-confocal system has the quality of optical sectioning.