Effect Of RNA Interference Targeting RhoA Gene On The Expression Of Extracellular Matrix Contents In HSC-T6

OBJECTIVE: To investigate the effect of RNA interference targeting RhoA gene on the expression of RhoA, Collagen typeⅠ(ColⅠ), Prollagen typeⅢ(PCⅢ), hyaluronic acid(HA)and laminin(LN)in rat hepatic stellate cell HSC-T6.METHODS: Transfect negative control plasmid vectors (named HK-A) and rat RhoA gene specific siRNA expressing plasmid vectors(named Rat1 and Rat2)into rat hepatic stellate cell HSC-T6 respectively. Divide the HSC-T6 cells into four groups as following: group A (cultured normally),group B (transfected with HK-A),group C (transfected with Rat1),group D (transfected with Rat2). The mRNA expression of RhoA, ColⅠand PCIII in HSC-T6 was detected by reverse-transcription polymerase chain reaction(RT-PCR)respectively in each group. The content of HA and LN in culture serum was measured by specific enzyme-linked immunosorbent assay (ELISA).RESULTS: Compared with group A, the expression of RhoA, ColⅠ, PCIII, HA and LN were decreased in group C (p﹤0.05 for RhoA and PCIII, the inhibition ratios were 43.77% and 43.68%. p﹤0.01 for ColⅠ, HA and LN , the inhibition ratios were 71.00%, 59.53% and 93.08% respectively). Otherwise, compared with group A, there was no significant difference in group B or group D for the five indexs (p﹥0.05).CONCLUSION: RNA interference mediated by Rat1 plasmids targeting RhoA can depress the expression of ColⅠ, PCⅢ, HA and LN in rat hepatic stellate cell HSC-T6, which has provided experimental foundation for further research in vivo.