Expression And Clinical Significance Of CGRP, SP And NPY In Adrenal Disease

OBJECTIVE:For reveal the the roles and clinical significance of Neurpeptide Y (NPY),substance P(SP) and Calcitoni rgene related peptide(CGRP) in the in adrenal gland,we study the distribution of NPY,CGRP,SP in adrenal gland by immunohistochemistry and statistics percentage of colour positive cell to compare difference among adrenocortical adenoma,adrenocortical hyperplasia, pheochromocytoma,adrenal medullary hyperplasia and normal adrenal.METHODS:To collect 75 conventional paraffin wax tissues of adrenocortical adenoma, adrenocortical hyperplasia,pheochromocytoma,adrenal medullary hyperplasia and normal adrenal from our hospital from December 1980 toDecember 2006 and normal adrenal corned from corpse.Every group is 15 cases.Immunohistochemical technique was applied to detect the expression of NPY,CGRP and SP in routinely processed tissue specimens from human adrenal neoplasm and hyperplasia.All tissues were fixed by formalin,its concentration is 10 percentage.Fixed time is between eighteen hours and twenty-four hours.Specimens were embedded by ozocerite and chip to Sum thickness.One specimen was HE stain,another was NPY, CGRP,SP immunoreactivity.Normal adrenal was positive contrast and PBS replaced first antibody was negative contrast.Positive matter was found in cytolymph and its colour was brown.Judgement standard is percentage of positive cell.The destination was to study the characteristic of NPY,CGRP and SP in adrenal by immunohistochemistry and statistics percentage of colouer positive cell to compare difference to adrenocortical adenoma adrenocortical hyperplasia, pheochromocytoma,adrenal medullary hyperplasia and normal adrenal.RESULTS:CGRP,SP immunoreactivity was regularly seen in adrenocortical adenoma, adrenocorticohyperplasia and normal adrenal cortex and there was a significant difference among the three(P=0.002,P=0.006),but neither in the pheochromocytoma,adrenal medullary hyperplasia nor in the normal adrenal medulla.CGRP,SP expression was higher in adrenocortical adenoma tissue than that of adrenocorticohyperplasia and normal cortex,the same with SP.Besides,higher CGRP,SP expression were present in adrenocorticohyperplasia group than those of normal adrenal cortex group.There also was a positive correlation between CGRP,SP positive cell and blood pressure in adrenocortical adenoma and adrenocorticohyperplasia(r=0.801,P=0.010;r=0.686,P=0.021;r=0.811,P=0.022; r=0.523,P=0.013).NPY positive cell were showed in pheochromocytoma,adrenal medullary hyperplasia and normal adrenal medulla and there was a significant difference among the three(P=0.018),but not both in the adrenocortical adenoma, adrenocorticohyperplasia and normal adrenal cortex.NPY expression was higher in pheochromocytoma tissue than that of adrenal medullary hyperplasia and adrenal medulla.Besides,higher NPY expression were present in adrenal medullary hyperplasia group than those of normal adrenal medulla group.There also was a positive correlation between NPY positive cell and blood pressure in adrenal medullary hyperplasia and adrenal Pheochromocytoma(r=0.856,P=0.031;r=0.792, P=0.009).CONCLUSIONS:1.Maybe CGRP and SP correspond to induces adrenal hormone release Besides,there was a positive correlation between CGRP,SP positive cell and blood pressure in adrenocortical adenoma and adrenocorticohyperplasia.The same with NPY for pheochromocytoma and adrenal medullary hyperplasia.2.CGRP,SP and NPY may play an important role in the and the development of adrenal disease.The expression of CGRP,SP and NPY change from low to high in the process from normal adranal to adrenal hyperplasia and adrenal tumor.CGRP,SP and NPY are functional and may represent the molecular basis of adrenal Pheochromocytoma,as well as a potential new target for the diagnosis and therapy of adrenal disease.3.Previous literature has proved that NPY secrete from adrenal chromaffin cell and our experiment also has proved NPY high expression in adrenal medulla disease,so, we think that maybe NPY is the another vasoconstriction substance except norepinephrine and adnephrin,which pheochromocytoma or adrenal medullary hyperplasia secreted.