| COX-2(cyclooxygenase) has been found in a lot of human malignant tumors including colon cancer, stomach cancer, esophageal cancer,ovary cancer, mammary cancer, cervix cancer, endometrial cancer. There is a positive relationship between COX-2 and the genesis and development of tumors. Tumor biology behaviors can be altered by inhibiting the synthesis of COX-2. However,there is no obvious relationship between the expression of COX-2 and the utility of the drug. Two different endometrial cancer cell line,RL95-2 and Ishikawa were used in this study to explore the effect of Celecoxib,a COX-2 inhibitor, and investigate the COX-2 inhibitor's modulation on COX-2 and p53 to different endometrial cancer line. We hope this result can be used as the theoretical basis in endometrial cancer.1..The effects of Celecoxib on the growth and apoptosis of endometrial cancer cell line,RL95-2 and IshikawaObjective: To explore the effects of Celecoxib on the growth and apoptosis of RL95-2 and Ishikawa cell. Methods: MTT was used to investigate the effects of Celecoxib on the growth of RL95-2 and Ishikawa cell. Each cell was divided into 5 groups, which were control group(culture solution),Celecoxib group (12.5umol/L, 25umol/L,50ummol/L,100umol/L,200umol/L) . The samples were observed for 24hr, 36hr and 48hr respectively. FCM was used to detect the cell apoptosis rate. Each cell was divided into 2 groups, which were control group(culture solution),Celecoxib group(100umol/L). All the groups were observed for 24hr. Results:Celecoxib had the effect on inhibiting both cell proliferation. The effect was relevant to the dose of the drug. In the group of 100umol/L and 200umol/L,the growth inbition is different between such group and the others(P<0.05).In the group of 200umol/L,the growth inhibition is different between RL95-2's and Ishikawa's(P<0.05). The Celecoxib group's apoptosis rate of each cell was higher than the control group. In RL95-2 cell line,the ealier stage apoposis rate of the control group was 0.54%,and the Celecoxib group's was 4.75%. The advanced stage apoptosis rate of the control group was 1.40% and the Celecoxib group's was 10.4%. In Ishikawa cell line,the ealier stage apoposis rate of the control group was 2.66%,and the Celecoxib group's was 3.25% . The advanced stage apoptosis rate of the control group was 6.25% and the Celecoxib group's was 7.85%.The change of the ealier stage and the advanced stage of the apoptosis rate are more obvious in RL95-2 cell line than in Ishikawa cell line.2. The effects of Celecoxib on the expression of p53 and COX-2 mRNA and on the expression of COX-2 protein in RL95-2 and Ishikawa cellObjective: To explore the the effects of Celecoxib on the expression of p53 and COX-2 mRNA and on the expression of COX-2 protein in RL95-2 and Ishikawa cell line .Methods:Each cell was divided into 2 groups, which were control group(culture solution),Celecoxib group (100umol/L).RT-PCR was used to detect the mRNA expression of p53 and COX-2 and Western Blotting was used to detect the protein expression of COX-2 in RL95-2 and Ishikawa cell after 24hr.Results: After 24hr, the COX-2 mRNA expression value of Celecoxib group was lower and the p53 mRNA expression value was higher than the control group in RL95-2. The COX-2 mRNA expression value of Celecoxib group was no obvious change and the p53 mRNA expression value was lower compared with the control group in Ishikawa. After 24hr, the COX-2 protein expression value of Celecoxib group was lower than the control group in RL95-2. The COX-2 protein expression value of Celecoxib group had no obvious change compared with the control group in Ishikawa.Conclusion: The results of this study pointed out: Celecoxib had the effect on inhibiting both cell proliferation. The effect was relevant to the dose of the drug. In the group of 100umol/L and 200umol/L,the growth inbition is different between such group and the others(P<0.05). In the group of 200umol/L,the growth inhibition is different between RL95-2's and Ishikawa's(P<0.05).Celecoxib could increase the apoptosis rate of both cells. The change of the ealier stage and the advanced stage of the apoptosis rate are more obvious in RL95-2 cell line than in Ishikawa cell line. In RL95-2 cell,Celecoxib can lower the mRNA expression of COX-2 and elevate the expression of p53. In Ishikawa cell,Celecoxib can elevate the mRNA expression of p53 and alter the expression of p53 unobviously. All of these indicated that COX-2 inhibitor can influence the cell's growth and apoptosis in COX-2 and non-COX-2 pathway. It is possible that p53 take an role in anti-tumor effect of it. This would be a promising new method for the therapy of endometrial caner.
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