Effects Of Transfected P21^waf1 Gene On The Duplication Of Centrosomes And The Proliferation Of Human Breast Carcinoma Cells

[Objective]To explore the role of transfected p21^waflgene in the duplication of centrosomes and the proliferation of human breast carcinoma cells.[Methods]p21^waflgene packaged with lipofectin was transfected into the cells of a human breast carcinoma cell line MCF-7,which stably expressed p21^wafl gene The cell growth was detected by using MTT.The cell cycle pattern was assayed by using flowcytometry.The duplication of centrosomes of breast carcinoma cells were detected by using indirect immunofluorescence microscopy.[Results]After transfection of pIRES-p21^wafl,The p21^wafl mRNA and protein of MCF-7-p21^waflwere highly expressed as compared with MCF-7 cells.Cell growth was obviously inhibited and resulted in an accumulation of cells in G₁,presenting that the proportion of the cells in G₁ phase was obviously increased from(49.84±1.58)%to (70.52±3.21)%,and that of the cells in S and G₂/M phases was obviously decreased from (36.83±1.36)%to(16.38±1.17)%and from(15.64±1.09)%to(9.42±0.98)%,resp -ectively, as compared with control group.The proportion of cells which contained unnormal centrosomes in MCF-7-p21^waflwere obviously decreased as compared with MCF-7 cells (P＜0.01).[Conclusion]The transfer of p21^wafl gene could inhibit the growth and the unnormal duplication of centrosomes of human breast carcinoma cells,which even could inhibit the genesis and development of breast cancer and might be become a new method to treat the breast cancer.