Expression And Significance Of Rho-associated Protein KinaseⅡ In Preeclamptic Placenta And Umbilical Artery

Objective To investigate the expression and significance of Rho-associated protein kinaseⅡ(RockⅡ) in preeclamptic placenta and umbilical artery.Methods Semiquantitative RT-PCR and Western blot were used to investigate the expression of RockⅡmRNA and RockⅡprotein in placenta and umbilical artery from 35 women with moderate preeclampsia (MPE group),38 women with severe preeclampsia (SPE group) and 45 normal third trimester pregnant women (normal pregnancy group).Results (1)The expression of RockⅡmRNA of placenta in MPE group(0.82±0.14)and SPE group(0.93±0.13) were significantly higher than that in normal pregnancy group(0.70±0.12)(P<0.01).The expression of RockⅡprotein of placenta in MPE group(0.79±0.15)and SPE group (0.92±0.12) were significantly higher compared with normal pregnant group(0.68±0.11) (P <0.01).The expression of RockⅡmRNA and protein of placenta in SPE group were higher compared with MPE group (P <0.01) .(2) The expression of RockⅡmRNA of umbilical artery in MPE group(0.69±0.13)and SPE group(0.52±0.12) were significantly lower than that in normal pregnant group(0.76±0.10)(P<0.01) .The expression of Rockll protein of umbilical artery in MPE group(0.68±0.10)and SPE group (0.51±0.12) were lower compared with normal pregnant group(0.75±0.13)(P<0.01).The expression of RockⅡmRNA and protein of umbilical artery in SPE group were significantly lower compared with MPE group (P <0.01) .(3)There were no correlation between the expression of Rockll mRNA and protein in umbilical artery with preeclamptic group and the S/D value and birth weight (P >0.05). Conclusion(1)The expressions of RockⅡmRNA and protein of preeclampsia group is upregulative in placenta and is downregulative in umbilical artery.(2)The varieties of the expressions of RockⅡmRNA and protein may be contributed to pathologic physiology progress in preeclampsia.