| Objective To investigate the expression of neurotensin(NT) and high-affinity NT receptor subtype 1(NTS1) in pancreatic cancer and clinical significance,and to explore the contribution of them to the growth of the pancreatic cancer cells PANC-1.Methods The method of immunohistochemistry was used to compare the expression of NT and NTS1 in pancreatic ductal adenocarcinoma,islet cell adenoma,chronic pancreatitis and normal pancreatic tissue and their interrelationship.Methyl thiazolyl tetrazolium(MTT) assay was conducted to examine the effect of different concentrations of NT and specific antagonist to NTS1,SR48692 alone or combined on the proliferation of PANC-1 cells.Result(1) The positive rate of NT was 87.5%(35/40),75%(15/20),50%(10/20),20%(2/10) respectively and NTS1 was 85%(34/40),20% (4/20),10%(2/20),0(0/10) respectively in pancreatic ductal adenocarcinoma,islet cell adenoma,chronic pancreatitis and normal pancreatic tissue.The positive rates of NT and NTS1 were significantly higher in pancreatic ductal adenocarcinoma than in chronic pancreatitis and normal pancreatic tissue.80%(32/40) of pancreatic ductal adenocarcinomas were positive for the coexpression of NT and NTS1,both of which obviously correlated.(2) Different concentrations of NT(10-10～10-7mol/L) significantly stimulated the growth of PANC-1 cells in a dose-dependent manner.Different concentrations of SR48692(10-11～10-7mol/L) alone had no effect on the growth of PANC-1 cells.Different concentrations of SR48692(10-10～10-6mol/L) inhibited the stimulatory effect of NT(10-9mol/L) in a dose-dependent fashion.Conclusion Because of their overexpression in the pancreatic cancer, NTS1 can be considered as a new marker for the diagnosis.Since NT stimulates the growth of PANC-1 via NTS1,NTS1 may serve as a new target to the therapy of the pancreatic cancer.And it is possible that the interaction of NT and NTS1 regulate the genesis and development of the pancreatic cancer.
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