The Effect Of Glucosidorum Tripterygii Tororum On The Expression Of Foxp3 Gene In Type 1 Diabetic Rat Model

Objective To investigate the change of Foxp3~+ regulatory T cells in type 1 diabetic rat model and the effect of Glucosidorum Tripterygii Tororum (GTT) on it, try to discuss the significance of the expressions of Foxp3 on the pathogenesy of type 1 diabetic.Methods Wistar rats were divided evenly into 3 groups by random. Diabetic model was developed by multiple low dose intraperitoneal injection of streptozotocin in groupâ…¡,â…¢, and groupâ… was treated as normal control. After the establishment of the model, administration of GTT was conducted in groupâ…¢, which was performed once a day, lasting three monthes. Execute a batch of rats every monthe to do the following experiment: segregate blood serum to detect the level of autoantibody by ELISA; do HE stain of pancreas to observe the pathological change; detect lymphocyte proliferation in spleen by lymphocyte transformation test (MTT assay) ; extract the total RNA in blood and spleen to investigate the quantitative expression of Foxp3 mRNA by Real-time fluorescent quantitative PCR method(Real-Time PCR); detect the expression of Foxp3 protein in lymphoid node and spleen by immunohistochemistry, then the results were compared with those of control group.Results The type 1 diabetic rat model was successfully developed by multiple low dose intraperitoneal injection of STZ. After development of the model, the blood glucose level in groupâ…¡,â…¢was obviously higher than that of control group (P< 0.01) , which lasted for three monthes. The pancreas associated autoantibody level in groupâ…¡,â…¢were significantly higher than that of groupâ… (P<0. 05) . HE stain of pancreas show lymphocyte infiltrating in pancreatic islets and matrix, the infiltrating degree in groupâ…¢was decreased than that of groupâ…¡after two monthes administration of GTT, which was even more obviously after three monthes treatment. The proliferation of splenic lymphocyte in groupâ…¡,â…¢was evidently increased comparing with the control group (P<0.01). In groupâ…¢the proliferation tended to breakdown in three monthes. As to groupâ…¡, it kept on the high level. In groupâ…¡,â…¢, the expressions of Foxp3 mRNA increased markedly compared with groupâ… (P< 0.05) both in blood and spleen. Moreover, the expression level of groupâ…¢was especially enhanced but compared with groupâ…¡, there was no significantly difference (P>0.05). During the three monthes, groupâ…¢show a tendency to increase but groupâ…¡descend. The expressions of Foxp3 protein in groupâ…¡,â…¢were augmented evidently contrast with groupâ… (P<0.05) , especially for groupâ…¢, however between groupâ…¡,â…¢, there are no statistical significance (P>0.05) .Conclusion Foxp3~+ regulatory T cells may involve in the pathogenesy of type 1 diabetes indused by STZ. Wether the immunoregulation effect of GTT on type 1 diabetes is performed by upregulating the expressions of Foxp3 in Tr is still need further research.