Studies On Isolation, Purification And Biological Activity Of Fruit Body Of Pleurotus Abalonus

Crude polysaccharides from the fruit body of Pleurotus abalonus were isolated by series of sequential steps including extraction with hot water,precipitation with 95% ethanol,removal of protein by Sevag method.The water-soluble polysaccharide(PPA-2) were obtained using DEAE Sephadex A-25 anion-exchange chromatography and Sephadex G-100 gel filtration chromatography.PPA-2 was homogeneous identified by polyacrylamide gel electrophoresis with a molecular mass of 23000.The infrared spectrum analysis showed that PPA-2 had polysaccharides specific absorbent peak,and the specific absorbent peak of nucleic acid and protein were not be found in the UV spectrum.A certain dosage of PPA-2 could inhibit the transplanted tumor growth of S₁₈₀, improve non-specific immune function and specific immune function such as humoral immunity,cellular immunity for mice.Vivo experiments showed that PPA-2(75 mg/kg,100 mg/kg) have antitumor activities on the implanted Sarcoma 180 of mice(P＜0.01).PPA-2(50 mg/kg,75 mg/kg, 100 mg/kg) could increase the spleen index in tumor-bearing mice(P＜0.01,P＜0.001). PPA-2(75 mg/kg,100 mg/kg) could increase the spleen index in normal mice(P＜0.05,P＜0.01).PPA-2(50 mg/kg,75 mg/kg,100 mg/kg) could increase the spleen index in lower immune mice(P＜0.05,P＜0.01,P＜0.001),and enhance the phagocytic rate(P＜0.05,P＜0.01) and the phagocytic index(P＜0.05,P＜0.001) of celiac macrophage, production of serum hemolysins IgM(P＜0.05) in normal mice.PPA-2(75 mg/kg,100 mg/kg) could enhance the function of delay hypersensitivity induced by DNFB(P＜0.05, P＜0.001) to normal mice.