| The genus Berchemia(Rhamnaceae) comprises about 31 species,occurring mainly in Asian,of which eighteen were distributed in the south of China.Its root was used as folk medicine to treat gall stones,stomach-ache,rheumatism and lumbago in China. The chemical constituents and bioactivities of Berchemia floribunda were studied, which was distributed in the Jianfengling of Hainan province,P.R.China.In the course of our search for active components from 95%ethanol extration of the roots of Berchemia floribunda,17 compounds were isolated by chromatographed columns of silica gel,ODS and sephadex LH-20.Their structures were elucidated by analysis of several spectra such as UV,IR,MS,1D-NMR and 2D-NMR,as floribundiquinone A-E(1-5),and 2-acetylphyscion(6),and eleven known compounds. 10-(chrysophanol-7'-yl)-10-hydroxychrysophanol-9-anthrane(7),physcion(8), chrysophanol(9),Helminthosporin(10),aloe-emodin(11),xanthorin(12),(2R, 3R)-3,3',5,5',7-pentahydroxyflavanone(13),lupeol(14),β-sitosteol(15),β-daucosterol(16),long chain fatty(17).Among these,compounds 1-6 are six new compounds,compounds 7,10-13,were first reported from genus Berchemia.The pharmacological experiment indicated significant hepatoprotective effects of EtOAc and n-Butanol fractions of 95%ethanol extracts against D-galactosamine-induced toxicity in WB-F344 cells,the availability of which was reached 38%at the concentration of 1×10-4M in vitro.Compounds 1-4,8 and 9,isolated from the EtOAc fraction,were also monitored,among them,compounds 3 and 4 showed potent hepatoprotective effect,which availability were 81.0%and 76.0%at the concentration of 1×10-4M in vitro,resepectively.Active compound was not found in pharmacological monitor experiment by MTT methods,which have cytotoxic activities against the tumor cell HCT-8,Bel-7402,BGC-823,A-549 and A2780.
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