Effect Of Fasudil On The Function Of Endothelial Cells Induced By Oxidized Low Density Lipoprotein

Objective To investigate whether fasudil can protect the function of cultivated human umbilical vein endothelial cells(HUVECs) induced by oxidized low density lipoprotein.Methods HUVECs were cultivated in four groups with different concentrations of fasudil (0μmol/L,1μmol/L,10μmol/L, 100μmol/L) respectively for 24hs and then the expression amount of eNOS in HUVECs of each group was detected by western-blotting. The HUVECs cultivated in vitro were divided into four groups which were treated by fasudil, oxLDL, fasudil + oxLDL, and normal culture medium respectively. After 24hs, the amount of eNOS in each group was detected by western-blotting. And the amount of the NO2-/NO3- , which is the metabolic product of NO and indicates the release amount of NO, was detected by the nitrate reductase assay (NRA).Result In a concentration depend manner, treatment with fasudil (1μmol/L, 10μmol/L, 100μmol/L) increased eNOS protein expression to 121±8﹪,147±13﹪,181±16﹪respectively than the contrast group (fasudil 0μmol/L).The differences between each group were significant, p<0.05. Compared with the basal level in group control, the eNOS protein level was significantly increased in group fasudil(171±15﹪) (p<0.01) and in group fasudil + oxLDL (133±11﹪) (p<0.01), decreased in group oxLDL(67±6﹪) (p<0.01). Compared with group contro(l48.12±5.34),the production of NO was significantly increased in group fasudil(72.52±6.21) (p<0.01), decreased in group oxLDL(26.26±4.54)(p<0.01)and in group fasudil + oxLDL(41.31±5.11) (p<0.05). Conclusion OxLDL decreased the production of eNOS and NO in cultivated human umbilical vein endothelial cells, while fasudil could reverse or partly rectify these effects. Fasudil could protect the function of cultivated HUVECs.