The Effect Of CRH On Phagocytosis Of Severe Trauma Rat Peritoneal Macrophage

Objective: Being one of the most important neurohormones and regulatory factors in the HPA axle (hypothalamic-pituitary-adrenocortical), CRH(corticotropin releasing hormone) has broad spectrum biological features, which involving in regulating the cerebrum, adrenal gland, reproductive system, and placenta as well as affecting the processes of stress, neuroendocrine, rhythmic, behavioural, inflammation, and psychotic diseases. It was reported that CRH could stimulate macrophages and mastocytes to secrete cytokines, which includes both anti-inflammatory and pro-inflammatory factors, depending on the type of CRH receptor. However, it has not been reported how CRH affects the phage function of macrophages. The author adopted ex vivo rat abdominal macrophages as cell model and severely traumatized rats as animal model, to observe the CRH effects on phage function in normal and post-trauma conditions and discuss the role of CRH in early post-trauma pathogenesis.Methods: Collecting normal control group and post-trauma group rats through lavage and culturing. Observing 1). CRH on activity of macrophages: after treating with CRH, recording the OD values of each group in different time periods; 2). CRH on phage function: after treating with CRH, observing the changes of ability of macrophages, from normal group and post-trauma group, engulfing chicken erythrocytes.Results:1. CRH could improve the activity of normal macrophages, and such an improvement has a direct correlation with concentration of CRH, which peaks at about 10^-9M.2. Activity of traumatized macrophages was increasing significantly in early phase of severe trauma, which peaks at 2hr post-trauma, especially after CRH-treating.3. CRH could strengthen phage function of normal abdominal macrophages, and such an effect has a time-dependent feature, which peaks at 2 hours post-treating and declines at 4hr post-treating.4. Trauma could push the phage peak time an hour earlier, while CRH treating could increase that effect significantly and declined 2 hours later. Conclusion:1 .CRH could improve the activity of normal macrophages; Activity of traumatized macrophages was increasing significantly in early phase of severe trauma, which could be increased further by CRH. It could be said that CRH might increase the activity of macrophages in early phase of trauma.2.CRH could strengthen phage function of normal abdominal macrophages, and trauma process could push that peak time 1 hour earlier; it could deduced that CRH could increase the phage function of macrophages post-trauma by observing the phenomenon that CRH treating could increase that effect significantly and declined 2 hours later.