The Triazole Compounds Induce Apoptosis On Tumour Cells

Triazole compounds have been found to show extensive biological activities. In this cxperiment,we synthesized triazole compounds to validate their activities in BEL-7402 cells, human breast cancer cells MCF-7 and rat breast cancer cells 4T-1.We synthouzed triazole compounds first, and their structures were confirmed by maans of 1HNMR, IR determination, of which one compound 4-ami-no-5-((4-ohentyl-lH-l,2,3-triazol-l-yl)methyl)-4H-l,2,4-triazole-3- thiol (APTMTT) was selected. It was demonstrated that APTMTT could signifcantly inhibit pioliferation of cultured hepatocarcinoma BEL-7402 cancer cells in a dose-and time-dependent manner. Treatment with APTMTT resulted in gene- ration of reactive oxygen species (ROS), disrup- tion of mitochondrial mem- brane potential (ΔΨm), DNA fragmentation, and ATPase activities in BEL -7402 cells..The results suggested thatΔΨm is the first thing in apoptosis, the loss ofΔΨm eoeurred prior to cell death , then ROS increasing, and at the same time , Bcl-2 and Bax releasing, which contribute to cell apoptosis.APTMTT also induce obvious growth inhibition in human breast cancer cells MCF-7 and rat breast cancer cells 4T-1. Treatment with APTMTT resulted in generation of reactive oxygen species (ROS), disruption of mitochondrial membrane potential in these cells, But no DNA-Ladder.It form specifically length DNA fragmentation.Summing-up, APTMTT could induce obvious growth inhibition and apoptosis in cancer cells. It can induce disruption of mitochondrial membrane potential, and activate albumens which can regulate cell apoptosis.This disquisition may be a forceful evidence for anti-cancer of triazole compounds.