Study On Electrochemical Behavior Of Hemoglobin In Copper Ions And Potassium Persulfate

Hemoglobin is a protein of transporting oxygen. It is the colored component of blood and keeps the balance of pH. The abnormal content of hemoglobin causes many diseases, so the determination of hemoglobin is an important part of clinical blood routine detection. This thesis studies the determination of hemoglobin by polarography wave, we found potassium persulfate can cataly hemogloin on the dropping mercury electrode, and in the presence of copper ions, the reducton wave of hemoglobin can be increased; at the same time, the sensitivity of hemoglobin can be further improved when potassium persulfate and copper ions in the presence together. Compared with the literature, their detection limit was more reduced, and these methods are simple,ecomomical.The study has three parts, the primary contents are described as follows:In chapter one, the determination of hemoglobin and potassium persulfate are studied by a parallel catalytic wave. In pH 9.5 NH3-NH4Cl buffer solution hemoglobin brings forth a small reductive wave at– 0.70 V (vs. SCE) by using single sweep polarography. This reductive wave can be catalyzed by oxidant potassium persulfate. The derivative peak height is linearly proportional to the concentration of hemoglobin in the range of 2.0×10-10 ⁵.0×10-8 mol/L. The detection limit is 1.0×10-10 mol/L. The proposed method has been applied to the determination of hemoglobin content in venous blood samples with satisfactory results. The mechanism study has showed that the polarographic wave is attributed to the oxidation of protoporphyrin (IX) of heme prosthetic group.In chapter two, it shows the polarographic reduction wave of hemoglobin and Cu²⁺. In pH 10.1 NH3-NH4Cl 2.2×10-4 mol/L Copper Sulfate supporting electrolyte, Hemoglobin in a single sweep polarography on in - 0.77 V (vs. SCE) have near a polarographic catalytic wave. The second-order derivative peak current of the polarographic catalytic wave with peak potential - 0.77 V (vs. SCE) was proportional to the Hemoglobin concentration in the range of 5.0×10-11 ¹.2×10-7mol/L. The detection limit is 1.0×10-11 mol/L. This method has been used to determine the hemoglobin in urine, the results was satisfactory. The production mechanism of polargraphic wave of hemoglobin was discussed. Chapter three discusses the behaviour of the polarographic wave of hemoglobin in compositing system of potassium persulfate and Cu²⁺. The new catalytic wave is 35 times than before. The derivative peak height is linearly proportional to the concentration of hemoglobin in the range of 4.65×10-12 2.48×10-7 mol/L. The detection limit was reduced.