Effects Of Hypoxic Preconditioning On The Expression Of PHD2 And HIF-1alpha In Retina Of Light Damage Mouse

Objective Hypoxic preconditioning can activate endogenous neuroprotective system to protect retinal light injury and has a bright future in the treatment of retinal disease. Present paper was to study the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and prolyl-hydroxylase 2 (PHD2) in the retina of light damage mouse and investigate the possibly protective pathogenesis of hypoxic preconditioning against it. Methods Fifty-four BALB/C mice were randomly divided into simple light exposure group, hypoxic preconditioning group and normal control group. The former two groups were subdivided into light exposure 6, 12, 36 hours groups and 7 days group. Expressions of HIF-1 alpha and PHD2 were detected by immunohistochemical staining, and the RT-PCR was adopted to detect the aim gene PHD2. Results No HIF-1 alpha, PHD2 positive cells were found in normal controlgroup. Expression of HIF-1 alpha protein was higher at four episode in hypoxic preconditioning group compared with simple light exposure group, while expression of PHD2 was lower at four episode in hypoxic preconditioning group compared with simple light exposure group, with a statistically significant difference (P<0.01). Conclusion Hypoxic preconditioning increases the expressions of HIF-1 alpha by inhibiting PHD2 to decrease the degeneration of HIF-1 alpha, and achieve the protection to retinal light injury.