Studies On Antitumor Secondary Metabolites From Mangrove Endophytic Fungi

In our study on the chemical constituents from endophytic fungi of mangrove plants,30 compounds were isolated from mangrove fungi Penicillium citrinum and Penicillium griseofulvum.So far 23 compounds were elucidated by the means of spectroscopic method(UV, IR,MS,1D and 2D NMR) and comparison of their physiccochemical properties with references: citrinin(1),decarboxydihydrocitrinone(2),(3R~*,4S~*)-6,8-dihydroxy-3,4,7-trimethyliso coumarin(3),phenol A(4),3,4,5-trimethyl-1,2-benzenediol(5),threo-4-(3-hydroxybutan-2-yl) -3,6-dimethylbenzene-1,2-diol(6),threo-4-(3-hydroxybutan-2-yl)-3-methyl-6-acetylbenzene-1, 2-diol(7),cyclocitrinol(8),neocyclocitrinol(9),uridine(10),ergosterol(11),ergosterol peroxide(12),(3β,5α,6α,7α,22E)-5,6-epoxyergosta-8,22-diene-3,7-diol(13),(3β,5α,6β,22E)-ergosta-7, 22-diene-3,5,6-triol(14),soyasapogenol B(15),citrinin H2(16),glycerol 1-9',12'-octadecadienoate (17),daucosterol(18),1-(2-furanyl)-1,2-ethanediol(19),6,8-dihydroxy-3, 4,5,7-tetramethylisochroman(20),(S)-3-(3,5-dihydroxy-2,4-methyl-phenyl)butan-2-one(21), scalusamide A(22),7,8,4'-trihydroxyisoflavone(23).Among them,compounds 3,6 and 7 were new compounds,compound 5 was a new natrual product,and compound 9 was isolated from Penicillium citrinum for the first time.Cytotoxic activities of some compounds were evaluated by MTT method in vitro,and the results showed that compounds 1,3,and 4 were active components.