| ObjectiveWe recorded the expression of secondary lymphoid-tissue chemokine (SLC),CCR7, analyzed the relationship with clinical pathology of pancreatic adenocarcinoma (PAC) and investigated the correlation with angiogenesis and lymphangiogenesis of PAC. We also analyzed the microvessel density (MVD) and microlymphatic vessel density (MLVD) in PAC, and investigated the correlation with the expression of SLC and its receptor CCR7 in PAC, and illuminated the effect in angiogenesis and lymphangiogenesis of PAC.MethodsThe tissue samples including PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were obtained from 30 patients with PAC. The expressions of SLC, CCR7, VEGF-C and VEGF-D proteins in these tissues were assayed by immunohistochemical staining. The expressions of SLC, CCR7, VEGF-C and VEGF-D mRNA in PAC were also investigated by reverse transcription polymerase chain reaction (RT-PCR) and fluorescence quantitative real-time PCR.MLVD marked by VEGFR-3 and MVD marked by CD34 were detected by morphometric analysis respectively, and we analyzed the relationship between MVD, MLVD with clinical pathology of PAC. We also assayed the relationship between the expression of SLC, CCR7 with MVD, MLVD in PAC.Results(1) In the immunohistochemical staining we found the SLC protein mainly located in the normal pancreatic cell envelopes and/or cytolymphs. In all the samples, the positive rates of SLC protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were respectively 16.7% (5/30), 43.3% (13/30), 76.7% (23/30) and 46.6% (14/30).(2) In the immunohistochemical staining we found the CCR7 protein mainly located in the cell envelopes and/or cytolymphs of PAC. The positive rates of CCR7 protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were respectively 76.7% (23/30), 66.7% (20/30), 30.0%(9/30) and 70.0% (21/30). The results of RT-PCR and fluorescence quantitative real-time PCR indicated The expression levels of CCR7 mRNA in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were higher than that in the normal pancreatic tissues (P<0.01).(3) The MVD and MLVD in PAC were detected by morphometric analysis respectively. The level of MVD in III-IV stages was higher than I-II stages of pancreatic cancer (P<0.01), and it has significant relationship with the differentiation and histology types of PAC (P<0.01). But it has no correlation between the lymphatic metastasis (P>0.05).(4) The level of MLVD in III-IV stages was higher than I-II stages of PAC (P<0.01), and in these cases which had lymphatic metastasis, the level of MLVD significantly increased (P<0.01). And there was no correlation between the differentiation and histology types of PAC (P>0.05).(5) There was 25 samples that the SLC protein was negative, and among these samples the MVD was higher than that in positive samples of SLC protein (P=0.012). And there was no significant correlation between the expression of SLC protein with MLVD of PAC (P>0.05).(6) There was 23 samples that the CCR7 protein was positive, and among these samples the MLVD was higher than that in negative group of CCR7 protein (P=0.004). These results indicated that the lower expression of SLC protein was significantly associated with angiogenesis of PAC, and the higher expression of CCR7 protein was significantly associated with lymphangiogenesis of PAC. (7) The expression levels of VEGF-C in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were higher than that in the normal pancreatic tissues (P<0.05), and its positive rates were respectively 73.3% (22/30), 66.7%(20/30), 63.3% (19/30). While in the the normal pancreatic tissues, the positive rates were only 43.3% (13/30). The positive rates of VEGF-D in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were respectively 56.7% (17/30), 50.0% (15/30) and 53.3% (16/30), and the positive rates in normal pancreatic tissues was only 26.7% (8/30), which was lower than that in PAC (P<0.05).(8) There were 19 samples which the expressions of CCR7 and VEGF-C protein were positive, and only 4 samples which the expressions of CCR7 and VEGF-C protein were negative. There was significant correlation between the expressions of CCR7 and VEGF-C protein in PAC which was tested by Spearman correlation test (P=0.038). It indicated that CCR7 and VEGF-C maybe promote the development of PAC mutually.Conclusions(1) The lower expression of SLC in PAC tissues was significantly associated with the increase of MVD, and SLC might participate in the regulation of angiogenesis of PAC.(2) The expression of CCR7 was significantly associated with lymphatic metastasis and TNM stages of PAC, and the higher expression of CCR7 in PAC tissues was significantly associated with lymphangiogenesis of PAC.(3) The MVD has significant relationship with the differentiation and histology types of PAC. The Lymphangiogenesis angiogenesis of PAC could be reflected by the detection of MVD. There was significant correlation between MLVD with TNM stages and lymphatic metastasis of PAC. The lymphangiogenesis of PAC could be reflected by the detection of MLVD.(4) There was significant correlation between the expressions of CCR7 and VEGF-C protein in PAC. CCR7 and VEGF-C maybe promote the lymphangiogenesis and lymphatic metastasis of PAC mutually.
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