Study On The Apoptosis-Inducing Effect Mechanism Of Baicalin On HepG-2 Cell Line

Objective: To study the inhibition proliferation and the apoptosis-inducing effect of Baicalin and the perhaps mechanism.Methods: The HepG-2 cell were cultured in vitro. To detect the change of the correlated index. The antiproliferation and cytotoxic efficiency of Baicalin on HepG-2 cell was assessed with MTT assay. The cell apoptosis effect was observed by inverted microscope, HE staining and scannin electron microscope (SEM). The cell growth cycle and apoptosis ratio were measured by flow cytometry (FCM). The expressions of caspase-3 protein was detected by Western blot.Results: (1) MTT assay disclosed that Baicalin inhibited the proliferation of HepG-2 cell and the effects were in a time-and-concentration dependent manner. (2) Inverted microscope, HE staining and SEM disclosed that Baicalin induced the typical apoptosis change in cell morphology. (3) FCM disclosed that the ratio of apoptosis was significantly different as compared with that of control group compared with the control group 5.47% ,deviation was significant (P<0.01) in 48 hours .the tumor cells on G2/M stage decrease obviously and on S stage, the cells increase notablely. (4) Immunocytochemistry disclosed bcl-2, p53 were significantly decreased and bax increased. (5) Western blot disclosed that followed by the increase of Baicalin, caspase-3 protein expression was step up gradually.Conclusions: Baicalin could make some effect on inhibiting proliferation and apoptosis- inducing on HepG-2 cell line, perhaps related to activating of Caspase-3.