High Concentration Of Glucose Inhibits CNP Inducel Smooth Muscle Relatation Of Rats Gastric Antrial

The C-type natriuretic peptides is natriuretic peptides family member country,and it was a peptide with 22 amino acid residues.CNP has been found in the Central nervous system,in the cardiovascular system,in the digestive system,in the reproductive system,in the pulmonary system and widely distributed almost all over the body.It has been known as a local regular factor to exert biological actions in the way of paracrine and autocrine and cell membrane natriuretic peptides receptors combine. activation GC produce in cell cGMPproduct difficulties.There have been three natriuretic peptides receptors(NPR),NPR-A NPR-B and NPR-C.The functions of CNP which are known at present are vasorelaxation,lowering blood pressure,anti-proliferation of vascular smooth muscle cells and cardiac fibroblasts,inhibition of hormonal secretions(LH,ANP, aldosterone),the activity of rennin-angiotensin,and inhibition of basal motility of rabbit colon and oviduct,and development of ovarian follicle, reductions in jejunal fluid and electrolyte secretion to keep electrolyte homeostasis.Additionally,CNP participate in gastrointestinal tract motion affect have studies make known.Our previous studies indicated that natriuretic peptide receptors were distributed in the gastric antral smooth muscles of rats,in cell cGMP product difficulties.High concentration of glucose is diabetic gastroparesis analysis of causes.But high concentration of glucose whether or not participate in in the gastric antral smooth muscles of rats don't know.In physiological ecology recording gauge working conditions,high concentration of glucose and CNP inhibits gastric mobility antrial smooth muscle of guinea-pig,Up to now.experiment observe high concentration of glucose and high osmose of mannitol CNP and SNP inhibits gastric antrial smooth muscle of guinea-pig in the tester group and model group of rats,and initial approach a subject from different angles in the machine-made.The results were as follows:1.Tester group(n=8,P＜0.05),tension an interest rate 33.64%±3.75% and inhibitory nerve an interest rate 11.95%±1.97%in the CNP1×10^-6mol/L, tension an interest rate 51.04%±3.78%and inhlbitory nerve an interest rate 21.59%±2.56%in the CNP3×10^-6mol/L,tension an interest rate 62.05%±4.52%and inhibitory nerve an interest rate 52.46%±6.21%in the CNP1×10^-7mol/L.High concentration of glucose(n=8,P＜0.05),tension an interest rate 20.41%±2.73%and inhibitory nerve an interest rate 4.11%±0.38%in the CNP1×10^-8mol/L,tension an interest rate 29.37%±2.56% and inhibitory nerve an interest rate 12.92%±2.87%in the CNP3×10^-6mol/L, tension an interest rate 43.29%±2.56%and inhibitory nerve an interest rate 35.44%±1.53%in the CNP1×10^-7mol/L.High osmose of mannitol(n=8, P＜0.05),tension an interest rate 15.42%±1.94%and inhibitory nerve an interestrate 9.71%±0.74%in the CNP1×10^-8mol/L,tension an interest rate 26.33%-+2.07%and inhibitory nerve an interest rate 18.19%±3.45%in the CNP3×10^-8mol/L,tension an interest rate 38.65%±2.44%and inhibitory nerve an interest rate 30.28%±2.52%in the CNP1×10^-7mol/L.2.Tester group(n=8,P＜0.05),tension an interest rate20.25%±1.87% and inhibitory nerve an interest rate 5.62%±0.33%in the SNP1×10^-8mol/L, tension an interest rate 30.71%±2.03%and inhibitory nerve an interest rate 12.79%±0.41%in the SNP3×10^-8mol/L,tension an interest rate 49.28%±4.52%and inhibitory nerve an interest rate 28.17%±3.46%in the SNP1×10^-7mol/L.High concentration of glucose(n=8,P＜0.05),tension an interest rate 2.36%±0.43%and inhibitory nerve an interest rate 1.56%±0.31%in the SNP1×10^-8mol/L,tension an interest rate 13.69%±2.17% and inhibitory nerve an interest rate 3.58%±0.72%in the SNP3×10^-8mol/L, tension an interest rate 20.53%±2.74%and inhibitory nerve an interest rate 6.53%±0.38%in the SNP1×10^-7mol/L.3.Model group(n=12,P＜0.05),tension an interest rate 34.29%±2.51% and inhibitory nerve an interest rate 17.57%±3.72%in the CNP1×10^-8mol/L, tension an interest rate 58.41%±3.04%and inhibitory nerve an interest rate 36.94%±3.83%in the CNP3×10^-6mol/L,tension an interest rate 68.63%±2.79%and inhibitory nerve an interest rate 57.71%±1.98%in the CNP1×10^-7mol/L.High concentration of glucose(n=12,P＜0.05),tension an interest rate 28.54%±1.03%and inhibitory nerve an interest rate 6.89%±2.33%in the CNP1×10^-8mol/L,tension an interest rate 37.67%±2.12% and inhibitory nerve an interest rate 18.22%±6.95%in the CNP3×10^-8mol/L, tension an interest rate 55.11%±3.96%and inhibitory nerve an interest rate 39.62%±4.37%in the CNP1×10^-7mol/L.4.Model group(n=12,P＜0.05),tersion an interest rate31.21%±2.17% and inhibitory nerve an interest rate 11.91%±0.42%in the SNP1×10^-8mol/L, tension an interest rate 42.05%±2.63%and inhibitory nerve an interest rate 24.86%±1.07%in the SNP3×10mol/L,tension an interest rate 58.17%±3.42%and inhibitory nerve an interest rate 35.29%±2.25%in the SNP1×10^-7mol/L.High concentration of glucose(n=12,P＜0.05),tension an interest rate 11.36%±1.58%and inhibitory nerve an interest rate 6.41%±0.13%in the SNP1×10^-8mol/L,tension an interest rate 25.43%±2.79% and inhibitory nerve an interest rate 18.71%±0.37%in the SNP3×10^-8mol/L, tension an interest rate 50.52%±4.04%and inhibitory nerve an interest rate 27.73%±0.69%in the SNP1×10^-7ol/L.The results presentation1.High concentration of glucose and high osmose of mannitol CNP and SNP inhibits gastric antrial smooth muscle of rats.Can osmotic change be interrelated。2.High concentration of glucose but CNP give rise to major factor in the diabetic gastroparesis.