The Study Of The Apoptotic Acute Leukemia Cells Induced By Chinese Medicine Compound FFJZ

Background:Leukemia is caused by the maglignant clone of the hemopoietic stem cells.It is one of the most common cancer and its morbidity is increased in recent year.The stagging of the differentiation and the blocking of apoptosis of leukemic cells are the two main mechanism of the leukemia development.All manner of leukemic cells can be inducted to terminal differentiation and apoptosis by miscellaneous drugs in vivo and vitro.Traditional Chinese drug has little side-effect.With the persistent improvement of knowing tumor,the traditional Chinese drug with apoptosis promotion of tumor cells has been given more and more attention.Objective:The study is to investigate the effect about apoptosis induction by Chinese medicine compound FFJZ and the efficinency of FFJZ synergy the chemotherapy,in order to know synergetic effect and attenuation of FFJZ and to provide experimental and clinic evidence to treatment of acute leukemia,and to search a method on treatment of acute leukemia by Chinese medicine and western medicine.Methods:1.Experimental study:(1)The growth states of leukemia cells K562 cultrued in vitro were observed by the invent microscope after cultured for 24,48,72 hours.(2)The number of live K562 cells were counted by trypan blue exclusion..(3)The vegetation of the cells K562 was estimated by MTT method.(4)FCM(flow cytometre methods) was used to assess the intracellular fluorescence intensity of Annexin V-FITC/PI,and to assess account of apoptotic cells.2.Clinical study:The 40 cases of acute leukemia were randomly divided into two groups.There were 20 cases in treatment group,treated with FFJZ plus chemotherapy;There were 20 cases in control group,treated with chemotherapy.The clinicial therapeutic effectiveness was observed between two groups.Result:1.Experimental study:(1)After 48 hours culture,the morphology of K562 cells that FFJZ's concentration is 1mg/ml and less than 1mg/ml became fusiform and claviform.The K562 cells that FFJZ's concentration is 2mg/ml has no conspicuous different.The K562 cells that FFJZ's concentration is 4mg/ml and 6mg/ml took on shapes of crenation.(2) The count of the live K562 cells in As₂O₃ group grew slowly after 24 hours'culture.It was also found in FFJZ 4mg/ml,6mg/ml and 8mg/ml group.There was scarcely live cells in FFJZ 10mg/ml group after 24 hours'culture.(3)By the MTT assay,we found that the K562 cell growth and proliferation of all the experimental group except the FFJZ 2mg/ml group were inhibited in different extents.The inhibitions were significant differences in contrast with the control group(P＜0.05),and the inhibition rate of 8mg/ml group was higher than that of other experimental groups(P＜0.05).There are no significant differences berween FFJZ 4mg/ml group and As₂O₃ group(P＞0.05).The ICS0 of K562 cells was about 5.6mg/ml.(4) The FCM results demonstrated that the expression of early apoptosis of K562 cells in FFJZ 4mg/ml,6mg/ml and 8mg/ml group increased and there were significant differences in contrast with the control group(P＜0.01).Early apoptosis rate decreased when FFJZ'concentration was 8mg/ml,and the cells of late apoptosis/necrosis increased remarkblely in contrast with control group(P＜0.01).2.Clincal study:(1)The effective rates of treatment group and control group are 75% and 45%,showing statistical difference(P＜0.05);(2)There are 8 cases getting Complete Remission(CR) in treatment group,and there are 6 cases in control group.Days of keeping CR shows no statistical difference afar observing one year(P＞0.05);(3)There are no statistical difference between two group of side effect(P＞0.05).Conclusion:FFJZ can induct K562 cells to apoptosis and can conspicuously synergy the chemotherapy.FFJZ may become a safe and effective synergist with low toxicity in acute leukemia chemotherapy.