| The dietary structure of human being changes with the social development, which has a close relationship with the health situation of mankind. In developing countries, the dietary structure has been changing into high caloric diet and more and more chronic non-infectious diseases occurred. These diseases become serious problems to people′s health in those countries. A mass of epidemiology investigation and animal experiments were carried out domestically and overseas, while little research was focused on the effect of feeding high caloric diet to animals in different ages with the systematic experiments. Therefore, using mouse fed with different high diet in different ages, the indexes such as growth and development, energy metabolism, the weight of liver and spleen, and the biochemical indexes in blood, were all investigated in this paper in order to confirm the investigation result of epidemiology and reveal the influence of high caloric diet on body health in different ages. The research will also give a support on the establishment of the scientific dietary structure.Also,probe new way into founding experimentation animal madel with high-sugar and fat diet. The main results were as follows:(1) The indexes such as ingestion amount, liver weight, FBG and TG in young and TG in adult were all lower than the control group when the test mice were fed with high-sugar diet in young age alone, while the body weight in aged was higher than the control. There was no obvious difference between the other physiological indexes in the three stages. The results showed that the ingestion amount, glucose metabolism and liver weight were influenced in short term, while the lipid metabolism was affected in long term, when the test mice were fed with high-sugar diet in young age. Meanwhile, these mice fed with high-sugar diet in young age, did not appear as hyperglycaemia and obesity, but the hyperglycaemia and obesity still occurred in these aged mice even they were fed with the basic diet hereafter. (2) The indexes such as the oxygen consumption and TC in young age, spleen weight in adult and TG in old age were all higher than the control group when the test mice were fed with high-fat diet in young age alone. Meanwhile, the ingestion amount in old age decreased obviously. As for the other indexes in the three stages, there were no remarkable differences. It showed that the high-fat diet fed in young age alone influenced the energy metabolism of the young mice in a short term while affected the immunity, lipid metabolism and ingestion amount in a long term and increased the TG both in young age and old age.(3) Fed with high-sugar and high-fat diet in young age alone, the indexes such as the liver weight and TC in young age, the FBG and body weight in old age, were all much higher than the control group, while the ingestion amount in young age, the spleen weight in adult, the liver coefficient and the SOD activity in aged were all lower than the control group. Therefore, the high-sugar and high-fat diet fed in young mice alone had a shortdated influence to the indexes such as the ingestion amount, liver weight, glucose metabolism and lipid metabolism and at the same time, had a long-term influence to the immunity, glucose metabolism and antioxygen ability, which lead the test mice suffering from hyperlipidemia in young age, hyperglycaemia, obesity and the decrease of the ability of clearing the free radical in old age.(4) Fed with the high-sugar diet in middle age and old age mice, the indexes such as the oxygen-consumption, FBG and ingestion amount were lower than the control group, while the body weight was much higher than the control. There was no obvious effect on the other indexes in the test mice. It showed that fed with the high-sugar diet in middle age and old age mice performed an obvious influence to the basal metabolism, glucose metabolism, body weight and ingestion amount, which lead the test mice suffering from obesity, instead of hyperglycaemia.(5) Fed with the high-fat diet in the aged mice, the oxygen consumption and the ingestion amount were decreased notably. There were no obvious changes in the other indexes in the experiment. It showed that the high-fat diet in aged mice had an obvious influence to the energy metabolism and ingestion amount and no obvious influence to hyperlipidemia.(6) Fed with the high-sugar and high-fat diet in the middle age and old age mice, the oxygen consumption and ingestion amount were lower than the control group while the body weight was much higher than the control. There were no obvious effects on the other indexes in this experiment. Therefore, the high-sugar and high-fat diet in aged mice reduced basal metabolism and ingestion amount, and lead the test mice obesity.(7) When fed with the high-sugar in all ages in mice, the body weight was higher than the control group and lower than that in the test mice which fed with high-sugar diet in young age alone or old age alone. The indexes FBG and ingestion amount were lower extremely than the control group, and there were obvious individual difference between the test mice in spleen. There were no obvious effects on the other indexes in this experiment. The results revealed that the high-sugar diet in mice in all ages improved the tolerance ability against high-sugar diet as well as the adjustability to the body weight in old age, while it brought the bad effects on the immunity in the mice.(8) Fed with the high-fat diet in all ages in mice, the oxygen consumption and ingestion amount decreased while the TG and TC increased sharply. The individual differences of spleen and liver were remarkable.These disclosed that that the high-fat diet for mice in young and old age reduced the basal metabolism, heightened TG and brought bad influence to the immunity in the test mice.(9) The body weight and TC of mice which fed with high-sugar and high-fat diet in young and old age were much higher than the control group, while FBG, ingestion amount and oxygen consumption were much lower than the control.The results indicated that the high-sugar and high-fat diet for mice reduced basal metabolism, brought hyperlipidemia and obesity instead of hyperglycemia, which were much remarkable than those in the mice fed with high-sugar diet alone or high-fat diet alone, or those in the mice fed with high-sugar diet in young age alone or in middle and old age alone.(10) Different kinds of high-energy diet had different effects in mice.High-sugar diet influenced the body weight, FBG, TG and liver weight of mice, while high-fat diet influenced the oxygen consumption, spleen, TC and TG notably. The effect of high-sugar and high-fat diet was not the simple addition of the influences made by high-sugar diet alone and high-fat diet alone.(11) The indexes such as the body weight, liver, spleen and TC all showed the obvious individual differences when the test mice in middle and old age were fed with the high- calorie diet. The results showed that the adaptabilty of mice to high- caloric diet is different.(12) In conclusion, the effects of the high-calorie diet fed in young age mice were more efficient than those of the high-calorie diet in middle and aged mice. There were farthing mice had adaptability to High-caloric diet. But fed propriety high-sugar diet to mice in every phase can decrease FBG and TG .It shows that propriety high-sugar diet can advance adaptability of mice.The influence of high-fat diet is more than high-sugar diet.Further research should be carried out on the phenomenon that appropriate dosage high-sugar diet reduced the FBG level in the test mice, which was significant to disclose the relationship between the dietary structure and the diabetes.
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