| Infantile anorexia is a widespread feeding-behavior disorderly disease in clinic and Severity harm growth and development of child. Recently twenty years, with the traditional Chinese medicine theory that is Spleen transformation lying in regulation rather than nourishment guiding, the domestic scholar widely apply the activate spleen-energy law that can recover the transport mechanism of spleen and stomach and regulate the function of spleen and stomach better and this law is effective to cure child anorexia, and they systematically studied the effect of the activate spleen-energy law on digestive and absorptive function, it is confirmed that the activate spleen-energy law have significant regulation effect on the action and secretion of gastrointestinal tract. Recently, we established the juvenile rat model with anorexia by imitating the major cause of children and found that the concentration of braingut petide related with ingestion regulation such as CCK in hypothalamus and periphery blood of the anorexy model was obviously. abnormal. Erbao Granules was effective on regulating the concentration of braingut petide related with ingestion regulation in periphery blood of the animal mode1 and regulating the spontaneous discharge frequency of hypothalamus appestat neurons. These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain.In previous study, we recorded the spontaneous discharge frequency of LHA and VMH neurons by using of the extracellular recording method. But using this method merely precisely reflect the spontaneous discharge frequency of individual neurons, failure to show the activity of integral encephalic region relatived with ingestion regulation and the relationship among every encephalic region, moreover, failure to illustrate the relationship between the activity of Neurons and body fluid signal.FMRI which occure in 1990'makes possibility of revealing the relationship between the activity of Neurons and body fluid signal in vivo.In some scholar'study used fMRI showed strong correlation between the activity of the hypothalamic neuron and the plasma insulin concentrations.So it is necessary that the observation in the integral activity of the hypothalamic appestat of anorexia children,the relationship among every encephalic region and the relationship between the activity of Neurons and body fluid signal by using fMRI which can help to further analyze the effect of YunPi FuFang on the hypothalamic appestat and lay favourable foundation for deeply researching the relationship between the effect of YunPi FuFang on the hypothalamic appestat and the regulation of braingut petide concentrations related with ingestion in periphery blood.Previous studys showed PVN, VMH and LHA of the hypothalamus play a central role in the regulation of feeding behavior.This study used fMRI to research the function differences that occur in three hypothalamic domain above mentioned in response to oral glucose intake between anorexia children and normal children , research the function differences that occur in three hypothalamic domain above mentioned in response to oral glucose intake in anorexia children between pre-treatment of YunPi FuFang and Post-treatment of YunPi FuFang. These research can explore the center mechanism of Infantile anorexia and the mechanism of YunPi FuFang on Infantile anorexia.Results:1. fMRI response observed after oral glucose ingestion. An activation of of the fMRI signal was observed in the PVN , VMH and LHA of all subjects after glucose ingestion and were a degression after reaching the maximum fMRI signal. The maximum activation response was all observed between 30 and 45 dynamic state . Although a a degression was observed after reaching the maximum fMRI signal,the Status that the fMRI signal return the baseline in observed 35 min was not observed.This result corresponds to the altered brain function in the hypothalamus of the obese subject in response to food intake in previous study.2. The difference of the fMRI signal in response to oral glucose intake between anorexia group and normal group.There were differences of the fMRI signal in response to oral glucose intake in PVN, VMH and LPH between anorexia group and normal group.In the PVN , a significant attenuation in the magnitude of the activation response was noted in the anorexia versus normal group (13.8 5±1.46 vs. 30.47±1.58%,P<0.01 comparing with normal group). In the VMH, a significant accentuation in the magnitude of the activation response was noted in the anorexia versus normal group (36.37±2.65 vs. 24.91±1.34%,P<0.05 comparing with normal group). In the LHA, a significant attenuation in the magnitude of the activation response was noted in the anorexia versus normal group (25.60±0.89vs.33.32±1.44%,P<0.05 comparing with normal group).3. The difference of the fMRI signal in response to oral glucose intake in anorexia group between pre-treatment and post-treatment.By compare the fMRI signal intensity in the hypothalamus of the anorexia group which was observed in pre-treatment with post-treatment, the result show that the difference of the fMRI signal intensity between pre-treatment and post-treatment exists. In the PVN, an accentuation in the magnitude of the activation response was noted in pre-treatment versus post-treatment (13.85±1.46%,25.48±1.96%,P<0.05 comparing with post-treatment). In the VMH, an attenuation in the magnitude of the activation response was noted in pre-treatment versus post-treatment (36.37±2.65%,24.15±1.81%,P<0.05 comparing with post-treatment). In the LHA, an accentuation in the magnitude of the activation response was noted in pre-treatment versus post-treatment(25.60±0.89%, 32.67±1.13%, P<0.05 comparing with post-treatment).Results:1. The accentuation of the fMRI signal intensity reflect the hyperactivity of the neurons in response to food intake (glucose solution)in the PVN,VMH and LHA of the hypothalamus, this result correspond with that the three domains play a key role in the regulation of energy intake and feeding behavior confirmed in previous study.2. The difference of the fMRI signal of the hypothalamus in response to oral glucose intake between anorexia group and normal group further confirm that morbidity of infantile anorexia may be closely correlated with impaired function of the PVN, VMH and LHA of the hypothalamus.3. After treatment of YuPi FuFang on anorexia group for four weeks , the result of the fMRI signal of the hypothalamus in response to oral glucose intake confirm that YunPi FuFang possibly regulate the function of the PVN and VMH of the hypothalamus to treat Infantile anorexia.
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