Study On Effect Of Combination Of Thalidomide And As₂O₃ On VEGF And P-gp Expression In AML

Objective: To investigate the effect of thalidomide and As203 on the secretion of vascular endothelial growth factor(VEGF) and Pgp expression of the cell of AML.Methods:The patients with AML and healthy people in contrast group were analyzed. Heparin- anticoagulated blood were centrifuged to obtain plasma then conserved in -20℃fridge. The patients with AML were analyzed. Heparinized marrow was taken, and putted into Ficoll-isopaque solution to purified BMMNC.BMMNC and HL-60/VCR were both cultured in the RPMI 1640 medium, placed in 5% CO2 saturation and 37℃to proliferation.Added VCR to the medium of HL-60/VCR to sustain drug resistance. Thalidomide and As₂O₃ was added to substrate respectively. At the same time, a contrast group was set up. The concentration of VEGF in upper liquid and plasma was examined with enzyme-linked immunosorbent assay (ELISA)and Pgp expression on cell membranes was analyzed by flow cytometry (FCM).Results : The plasma VEGF concentrations in the patients with AML were significantly higher than the levels of the control (P<0.01).After added thalidomide and As₂O₃ to substrate, the concentration of VEGF in upper liquid and plasma is significantly lower than the contrast group.After added thalidomide and As₂O₃ to substrate, Pgp expression of the cell of AML and HL60/VCR was not significantly changed.Singnificant correlation was observed between P-gp and VEGF in newly diagnosed AML cases.Conclusions:Leukemia cell can secrete VEGF self;Both thalidomide and As₂O₃ can inhibit the secretion of VEGF of AML and HL-60/VCR;Both thalidomide and As₂O₃ can not affect the Pgp expression on the cell membranes of AML and HL-60/VCR.Test of the two targets together is more effective to the judgment of prognosis in AML.